Setting of seeds during Agrobacterium-mediated winter wheat transformation by in planta method

DOI: https://doi.org/10.7124/FEEO.v34.1631
Keywords: Triticum aestivum, Agrobacterium-mediated transformation, seed setting

Abstract

Aim. To investigate the frequency of seeds setting and the formation of transgenic plants during Agrobacterium-mediated transformation of winter wheat by the in planta method using different methods of applying agrobacteria and the composition of inoculation media. Methods. Agrobacterium-mediated transformation by in planta method, molecular genetic analysis, methods of mathematical statistics. Results. A significant difference was found in the studied genotypes of winter wheat according to the indicator seed setting depending on the A. tumefaciens strain used and the inoculation medium. When comparing the effect of different strains of agrobacteria on this indicator, no significant differences between them were established, however, a tendency to increase the frequency of seed formation was observed when using the LBA4404 strain. When using the MS-22 inoculation medium, which additionally contained 12.5 mM MES, 4 mM NH4CI, 5.5 mM MgSO4, a certain increase in the number of formed seeds was also observed compared to the MS-21 medium supplemented with sodium thiosulfate, the frequency of seed setting increased in on average by 5 %. Conclusions. The genotypic dependence of the frequency of seed setting and the formation of transgenic plants with the use of different inoculation media and the method of the transformation procedure was established.

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