Resistance of cellular variants of Nicotiana tabaccum L. plants to water stress

DOI: https://doi.org/10.7124/FEEO.v34.1628
Keywords: cell selection, tobacco, water deficit, Ba2 ions, stress, resistance, proline

Abstract

Aim. It has been established that resistance to some heavy metal cations is combined with resistance to osmotic stress. Thus, resistance to barium cations correlates with resistance to water stress. Therefore, the aim of the experiment was to compare the resistance of primary and secondary calli to modelled stresses. Methods. The object of testing was tobacco, a plant sensitive to water deficit. The level of resistance of the variants was assessed by the relative increase in fresh weight under osmotic stresses: (salinity, 25.0 g/l of sea water salts; sodium sulphate, water stress). Both concentrations are lethal for wild-type tobacco cell cultures. Results. Вa-resistant tobacco cell cultures were obtained. Tobacco callus were resistant to lethal modelled stresses. The Ba-resistant culture was developed on medium with the addition of sea water salts and sodium sulphate. Conclusions. The phenomenon of resistance was selected as a result of primary selection on the medium with heavy metal ions. The level of osmotic resistance did not decrease with increasing cultivation time.

References

Imran Q. M., Falak N., Hussan A., Man B.-G., Yun B.-W. Abiotic stress biotechnological tools in stress response. J. Agronomy. 2021. 11 (8). Р. 15–79. doi: 10.3390/agronomy11081579.

Kazemsouve M., Hatamian M., Tesfamaniam T. Plant dround stage influences heavy metal accumulation in leafy vegetables of garden cress and sweet basil. Chemical and biological technologies in Agriculture. 2019. 25 (6). Р. 1–16. doi: 10.1186/s40538-019-0170-3.

Petruzelli G., Pedrom F. Influence of increasing tungsten concentrations and soil characteristics on plant uptake: greenhouse experiments with Zea mays L. Appl. Sci. 2019. 9. 3998. Р. 22–45. doi: 10.3390/app.9193998.

Ahmed S., Ahmed S., Roy S. K., Woo S. H., Sonawane D., Shohael A. M. Effect of salinity on the morphological, physiological and biochemical properties of lettuce (Lactuca sativa L.) in Bangladesh. Open Agriculture. 2022. 4. P. 361–373. doi: 10.1515/opag-2019-0033.

Norouzi O., Hesami M., Pepe M., Dutta A., Maxwell A., Jones P. In vitro plant tissue culture as the fifth generation of bioenergy. Scientific Reports. 2022. 12 (5838). P. 1–11. doi: 10.1038/s41598-022-09066-3

Angulo-Bezarano P. I., Puente-Rivera J., Cruz-Orteza R. Metal and metalloid toxicity in plants: an overview on molecular aspects. Planta. 2021. 10 (4). P. 35. doi: 10.3390/plants/0040635

Slemi N., Kouki R., Ammar M. H., Ferrera R., Perez-Clemete R. Barium effect on germination, plant growth and antioxidant enzymes in Cucumis sativus L. Food science and nutrition. 2021. 9 (4). P. 2086–2094. doi: 10.1002/fsn3.2177.

Kibria M. G., Hoque Md. A. A review on plant responses to soil salinity and amelioration strategies. Open Journal of soil science. 2019. 9 (11). P. 1–32. doi: 10.4236/ojss.2019.911013.

Paes de Meloi B., Avelar Carpinetti P., Fraga O. T., Fiores V. S., De Camargos L. F., De Silva Ferreiro M. F. Abiotic stress in plants and their markers: a practice view of plant stress and programmed cell death mechanisms. Plants. 2022. 11 (9). 1100. doi: 10.3390/plants1191100.

Colin I., Ruhnow F., Zhu L.-K., Zhao Y., Person S. The cell biology of primary cell watts during salt stress. Plant Cell. 2023. 35 (1). P. 201–207. doi: 10.1093/plcel/koa292.

Sergeeva L. E., Mykhalska S. I. Cell selection with heavy metal ions for obtaining salt tolerant plant cell cultures. Plant physiology and genetics. 2019. 51 (4). P. 315–323. doi: 10.15407/frh2019.04315.

Khan J., Laboni A., Ajim T., Rlias S. M., Seraj V. Downregulation of the OsAKT1 K+/Na+ transporter by CRISPR – Cas9 mediated transformation in sensitive rise IR29 makes it tolerant to salt stress. Plant tissue Culture and Biotechnology. 2023. 33 (1). P. 71–84. doi: 10.3329/pteb.v33i1.6668.

Peng J., Tian M., Cantillo N. M., Zawodzinski T. The ion and water transport properties of K+ and Na+ form perfluorosulfonic acid polymer. Electrochimica Acta. 2018. 282. P. 544–554. doi: 10.1016/j.electacta.2018.06.035.

Dragwidege J. M., Ford B. A., Ashnest J. R., Gendall A. Two endosomal NIIX – type Na+/H+ antiporter are involved in auxin-mediated development in Arabidopsis thaliana. Plant Cell Physiol. 2018. 59 (8). P. 1660–1669. doi: 10.1093/pep/pcy090.

Norouzi O., Hesami M., Pepe M., Dutta A., Jones A. M. P. In vitro plant tissue culture as the fifth generation of bioenergy. Scientific Reports. 2022. 15. 50 p. doi: 10.1038/s41598-022-09066-3.