Features of Usp1 expression and localization in different types of malignant neoplasms

DOI: https://doi.org/10.7124/FEEO.v34.1622
Keywords: USP1, UAF1 cofactor, CML, prostate cancer, USP1 expression, USP1 subcellular localization, tumorigenesis

Abstract

Aim. Malignant neoplasms pose a significant challenge in medicine, requiring a deep understanding of the molecular mechanisms underlying their pathogenesis. This study aimed to investigate the expression profile and subcellular localization of ubiquitin-specific protease 1 (USP1) in various types of cancer. Methods. Using the TCGA database, an analysis of USP1 expression was conducted across different cancer types. Immunofluorescence analysis with confocal microscopy was utilized to determine the subcellular localization of USP1 in chronic myeloid leukemia (CML) and prostate cancer cells. Results. Bioinformatic analysis revealed heterogeneous USP1 expression, with increased levels observed in acute myeloid leukemia, esophageal carcinoma, and glioblastoma multiforme. Immunofluorescence analysis confirmed nuclear localization of USP1 in CML cells and showed that inhibiting USP1 interaction with UAF1 using ML323 disrupts its transport to the nucleus. Prostate cancer cells exhibited both nuclear and atypical cytoplasmic USP1 localization, suggesting alterations in its expression, interaction with UAF1, and disruption of signaling pathways. Conclusions. USP1 plays a multifaceted role in cancer processes, with dysregulation of its expression and localization associated with tumorigenesis. Targeting USP1 holds therapeutic potential for cancer treatment.

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