Identification of molecular markers linked to leaf rust resistance gene Lr13 in wheat

  • O. V. Halaiev Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-7247-2910
  • M. V. Halaieva Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-8133-5184
DOI: https://doi.org/10.7124/FEEO.v34.1616
Keywords: bread wheat (Triticum aestivum L.), near-isogenic lines, leaf rust, Lr13, microsatellite markers

Abstract

Aim. Identification of codominant molecular markers closely linked to the Lr13 leaf rust resistance gene. Methods. PCR with detection in a polyacrylamide gel. Hybridological analysis. Phytopathology evaluation. Statistical analysis. Results. PCR analysis of varieties carrying the Lr13 gene and near-isogenic lines of the Thatcher variety with genes for resistance to leaf rust Lr13 (TcLr13), Lr16 (TcLr16), Lr23 (TcLr23) and the F2 population TcLr13 x TcLr34 by the microsatellite loci Xbarc13, Xbarc55, Xgwm148, Xwmc261, Xgwm271, Xwmc272, Xwmc344, Xbarc361, Xgwm410, Xwmc474, Xwmc477 and Xgwm630 was performed. To test the two microsatellite loci Xgwm148 and Xwmc344 as candidate markers for the Lr13 gene, we used the F2 population, which was obtained from crossing two near-isogenic lines of the Thatcher variety with leaf rust resistance genes Lr13 (TcLr13) and Lr34 (TcLr34). Conclusions. When comparing the genotyping data of F2 hybrids and the phenotypic manifestation of resistance to leaf rust, it was determined that the microsatellite locus Xwmc344 is closely linked to the Lr13 gene.

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