Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene

  • A. G. Komisarenko Institute of Plant Physiology and Genetics of NAS of Ukraine, Ukraine, 03022, Кyiv, Vasylkivska str., 31/17 https://orcid.org/0000-0003-2081-4055
  • S. I. Mykhalska Institute of Plant Physiology and Genetics of NAS of Ukraine, Ukraine, 03022, Кyiv, Vasylkivska str., 31/17
DOI: https://doi.org/10.7124/FEEO.v32.1544
Keywords: winter wheat, transgenic plants, proline, osmotic resistance, structural analysis of yield

Abstract

Aim. To investigate the level of tolerance to water deficit of seed generations (T1 and T4) of genetically modified winter wheat with partially suppressed expression of the proline dehydrogenase gene (ProDH) based on the analysis of physiological and biochemical indicators and economic characteristics of plants. Methods. Determination of indicators of crop structure and content of free L-proline (Pro). Results. The level of Pro was studied and the main elements of productivity in the offspring of transgenic plants and their original forms under normal and insufficient water supply were analyzed. Conclusions. T1 and T4 biotechnological plants under normal/stress conditions accumulated more Pro than the original genotypes. The level of this amino acid in genetically modified seedlings under optimal cultivation conditions exceeded the initial forms by an average of 1.8 times. Under the influence of water deficit, its content increased by 2.2 and 2.3 times, in relation to normal water supply. In non-transgenic variants, the level of Pro, under similar growing conditions, was lower by 1.9 and 2.0 times, compared to T1 and T4 plants. Under the optimal water regime, the analyzed wheat variants did not differ significantly in terms of productivity. Drought led to their decrease, but the offspring of biotechnological plants were characterized by a less pronounced difference, as well as a higher yield.

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