The response of pea plants to ultraviolet B radiation and cytokinin

  • V. V. Zhuk Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148 https://orcid.org/0000-0003-1966-7537
  • A. N. Mikheev Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148
  • L. G. Ovsyannikova Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148
DOI: https://doi.org/10.7124/FEEO.v32.1541
Keywords: UV-B, Pisum sativum L., BAP, photosynthetic pigments, HP

Abstract

Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content of photosynthetic pigments and hydrogen peroxide (HP) in leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B at a dose of 3.5 kJ/m2 per day during 5 days. Groups of the irradiated and non-irradiated plants were treated with 6-BAP after the end of the UV-B radiation. Length of shoots and roots of pea plants were measured during all time of experiment. Content of photosynthetic pigments and HP in leaves were determined during the experiment. Results. It was shown that plant growth delayed, content of photosynthetic pigments reduced, content of HP increased in the leaves after the chronic UV-B radiation of pea plants. The content of pigments increased and content of HP stabilized in pea leaves after the treatment of plants with 6-BAP. Conclusions. It was shown that chronic UV-B radiation of pea plants by dose of 3.5 kJ/m2 per day caused inhibition of plants growth, increasing HP content, decreasing photosynthetic pigments content in mature leaves. The BAP treatment of irradiated plants stimulated the restoration of photosynthetic pigments content in leaves.

References

Santin M., Ranieri A., Castagna A. Anything new under the sun? An update on modulation of bioactive compounds by different wavelengths in agricultural plants. Plants. 2021. Vol. 10. P. 2–38. doi: 10.3390/plants10071485.

Balcerowicz M. Anсient sun protection: the evolutionary origin of plant UB-signaling. Plant Physiol. 2022. Vol. 188. P. 29–31. doi: 10.1093/plphys/kiab517.

Moricony V., Binkert M., Costigliolo C., Sellaro R., Ulm R., Casal J.J. Perception of sunflecks by the UV-B photoreceptor UV RESISTANCE LOCUS 8. Plant Physiol. 2018. Vol. 177. P. 75–81. doi: 10.1104/pp.18.00048.

Qian M., Rosenqvist E., Prinsen E., Peschesk F., Flygare A. M., Kalbina J., Jensen A. K., Strid A. Downsizing in plants- UV-B light induces pronounced morphological changes in the absence of stress. Plant Physiol. 2021. Vol. 187. P. 378–395. doi: 10.1093/plphys/kiab262.

Zioutopoulou A., Patitaki E., O’Donnel L., Kaiserli E. Low fluency ultraviolet-B promotes ultraviolet resistance 8-modulated flowering in Arabidopsis. Frontiers in Plant Science. 2022. Vol. 13. P. 1–11. doi: 10.3389/fpls.2022.840720.

Jin H., Zhu Z. Dark, light, and temperature: key players in plant morphogenesis. Plant Physiol. 2019. Vol. 180. P. 1793–1802. doi: 10.1104/pp.19.00331.

Roeber V. M., Bajaj J., Rohde M., Schmulling Th., Cortleven A. Light acts as a stressor and influences abiotic and biotic stress responses in plants. Plant Cell Environ. 2021. Vol. 44. P. 645–664. doi: 10.1111/pce.13948.

Zhang R., Huang G., Wang L., Zhou Q., Huang X. Effects of elevated ultraviolet-B radiation on root growth and chemical signal-ing molecules in plants. Ecotoxicology and Environmental Safety. 2019. Vol. 171. P. 683–690. doi: 10.1016/j.ecoenv.2019.01.035.

Kataria S., Guruprasad K. N. Interaction of cytokinins with UV-B (280-315 nm) on expansion growth of cucumber cotyledons. Horticult. Int. J. 2018. Vol. 2. P. 45–53. doi: 10.15406/hij.2018.02.00025.

Vanhaelewyn L., Prinsen E., Van Der Straeten D., Vandenbusche F. Hormone-controlled UV-B responses in plants. J. Exp. Bot. 2016. Vol. 67. P. 4469–4482. doi: 10.1093/jxb/erw261.

Zhuk V. V., Mikhteyev A. N., Ovsyannikova L. G. The pea development after UV-B irradiation. Modern Phytomorphology. 2017. Vol. 11. P. 111–116. doi: 10.5281/zenodo.1050465. [in Ukrainian]

Zhuk V. V., Mikhteyev A. N., Ovsyannikova L. G. The photomorphogenetic reaction of pea plants (Pisum sativum L.) on ultra-violet irradiation effect. Factors in experimental evolution of organisms. 2017. Vol. 20. P. 179–183. [in Ukrainian]

Zhuk V. V., Mikhteyev A. N., Ovsyannikova L. G. Effect of chronic ultraviolet B radiation of pea plants. Factors in experi-mental evolution of organisms. 2022. Vol. 30. P. 67–72. doi: 10.7124/FEEO.v30.1463. [in Ukrainian]

Lichtethaler H. K. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol. 1987. Vol. 148. P. 350–382. doi: 10.1016/0076-6879(87)48036-1.

Chen L. M., Kao C. H. Effect of excess copper on rice leaves: evidence for involvement of lipid peroxidation. Bot. Bull. Acad. Sin. 1999. Vol. 40. P. 283–287.