Investigation of the influence of avermectin-containing preparations on the resistance of tomato lines to fusarium blight in vitro

DOI: https://doi.org/10.7124/FEEO.v32.1539
Keywords: Solanum lycopersicum, Streptomyces avermitilis, avermectin, Fusarium oxysporum f. sp. lycopersici, Fusarium solani

Abstract

Aim. Studying of the influence of avermectin-containing polyfunctional preparations Avercom and Avercom Nova on the enhancement of the resistance to fusarium wilt of tomato plants in vitro. Methods. Tomato plants were cultivated in vitro for 30 days in the presence of 25 μl/l of preparations Avercom and Avercom Nova. Analysis of the resistance of tomato plants to fusarium wilt was carried out by spraying with a conidia suspension of Fusarium oxysporum f. sp. lycopersici and F. solani. Resistance to fusarium wilt has been evaluated for 15 days after inoculation with the use of a 9-point scale. Results. It was established that cultivation in vitro of tomato plants cv. Money Maker in the presence of preparations Avercom and Avercom Nova at concentration 25 μl/l enhances the resistance of the selected plants to fusarium wilt caused by F. oxysporum f. sp. lycopersici and F. solani. In particular, resistance to these phytopathogens increased on average from 4 to 6 points on the 10th day after inoculation with F. oxysporum f. sp. lycopersici, and from 2 to 6 points on the 6th day after inoculation with F. solani. Conclusions. The results of the study show the enhancement of the resistance of tomato plants grown in the presence of Avercom and Avercom Nova preparations to the causative agents of the tomato fusarium wilt which indicates the prospects and promising opportunities of the use of microbial biopreparations for the protection of plants against biotic stress.

References

Agrios G. N. Plant Pathology. 5th ed. San Diego : Elsevier Academic Press, 2005.

Hariharan G., Sivasubramaniam N., Prasannath K. Chapter 6. RNA interference as a promising strategy for plant disease management. In: Kumar A., Droby S. (editors) Food Security and Plant Disease Management. Elsevier: Woodhead Publishing; 2021. P. 95–126. doi: 10.1016/B978-0-12-821843-3.00008-8.

Kolomiiets Y. V., Grygoryuk I. P., Butsenko L. M., Kalinichenko A. V. Biotechnological control methods against phytopathogenic bacteria in tomatoes. Appl. Ecol. Env. Res. 2019. Vol. 17. P. 3215–3230. doi: 10.15666/aeer/1702_32153230.

Iutynska G. O., Biliavska L. O., Kozyritska V. Y. Development strategy for the new environmentally friendly multifunctional bioformulations based on soil streptomycetes. Microbiol. Jour. 2017. Vol. 79 (1). P. 22–33. doi: 10.15407/microbiolj79.01.022.

Biliavska L. O., Kozyritska V. Ye., Valagurova Ye. V., Iutynska G. O. Biologically active substances of preparation Avercom. Microbiol. Jour. 2012. Vol. 74. P. 10-15. [in Russian]

Tsyhankova V. A., Andrusevych I. V., Biliavska L. O., Kozyrytska V. I., Iutynska H. O., Halkin A. P., Halahan T. O., Boltovska O. V. Growth stimulating, fungicidal and nematicidal properties of new microbial substances and their impact on si/miRNA synthesis in plant cells. Microbiol. Jour. 2012. Vol. 74 (6). P. 36–45. [in Ukrainian]

Kolomiiets Yu. V., Grygoryuk І. P., Butsenko L. М., Boroday V. V. Antifungal activity of the representatives of epiphyte microflora of the tomato seeds. Materials of the IV International scientific-practical conference of young scientists and specialists “Selection, genetics and technologies of the growth of agriculture plants, (Tsentralne village, 21 April 2016). 2016. P. 57–58. [in Ukrainian]

Podgorskiy V. S, Kotsoflyak О. I., Kiprianova Ye. А., Gvozdyak О. R. (editors). Ukrainian collection of microorganisms. Catalog of cultures. 2 ed. Kyiv : Nauk. Dumka, 2007. 270 p. [in Ukrainian]

Tkachyk S. O. (editor). Methodology of the phytopathological studies under conditions of artificial infection of plants. Kyiv : Nilan-LTD, 2014. 76 p. [in Ukrainian]

Buziashvili A., Cherednichenko L., Kropyvko S., Yemets A. Transgenic tomato lines expressing human lactoferrin show increased resistance to bacterial and fungal pathogens. Biocat. Agric. Biotechnol. 2020. Vol. 25. 101602. doi: 10.1016/j.bcab.2020.101602.

Tsutomu A., Hideki T., Motoichiro K., Tohru T. Tomato as a model plant for plant-pathogen interactions. Plant Biotechnol. 2007. Vol. 24 (1). P. 135–147.

Abbasi S., Naser S., Sadeghi A., Shamsbakhsh M. Streptomyces strains induce resistance to Fusarium oxysporum f. sp. lycopersici Race 3 in tomato through different molecular mechanisms. Front. Microbiol. 2019. Vol. 10. 1505. doi: 10.3389/fmicb.2019.01505.

Passari A. K., Upadhyaya K., Singh G., Abdel-Azeem A. M., Thankappan S., Uthandi S. et al. Enhancement of disease resistance, growth potential, and photosynthesis in tomato (Solanum lycopersicum) by inoculation with an endophytic actinobacte-rium, Streptomyces thermocarboxydus strain BPSAC147. PLoS ONE. 2019. Vol. 14 (7). e0219014. doi: 10.1371/journal.pone.0219014.

Blyuss K., Fatehi F., Tsygankova V. A., Biliavska L., Iutynska G., Yemets A., Blume Ya. RNAi-based biocontrol of wheat nematodes using natural poly-component biostimulants. Front. Plant Sci. 2019. Vol. 10. 483. doi: 10.3389/fpls.2019.00483.