In vitro culture of Zingeria biebersteiniana (Claus) P. Smirn. as a method for conservation and restoration of genetic diversity
Abstract
Aim. The aim of the work was to determine the conditions for development of in vitro culture, induction of callus formation, and long-term tissue culture of Zingeria biebersteiniana (Claus) P. Smirn. Methods. In vitro clonal propagation, tissue culture techniques. Results. The seed germination rate was found to increase significantly after long-term cold stratification. The protocol for seed sterilization was developed, which yielded 57.3% of aseptic plants. Collections of in vitro and pot cultured plants were created. Experiments on the adaptation of in vitro propagated plants to pot culture conditions revealed a high level of their survival. The optimal medium for in vitro clonal propagation was MS, supplemented with 0.1 mg/L NAA; while the most effective media for induction of callus formation and for long-term tissue culture was B5 supplemented with 2 mg/L 2,4-D and 0.1 mg/L BAP. Conclusions. The protocols and conditions for seed germination, in vitro clonal propagation, induction of callus formation, as well as long-term tissue culture of Z. biebersteiniana have been developed. The developed techniques of in vitro culture can be used for conservation and restoration of genetic diversity of the species, as well as to obtain sufficient plant material for further studies.
References
Tzvelev N.N. Zlaki SSSR. Leningrad: Nauka Publishers, 1976. 341 p. [in Russian]
Red Book of Ukraine. Plant Kingdom. Ed. Ya.P. Didukh. Кyiv: Global Konsalting, 2009. 900 p. [in Ukrainian]
Golubev V.N. “Pulsating” elements of regional floras. Bull. Nikit. Bot. Garden. 2004. Is. 90. P. 8–12. [in Russian]
Nikiforov A.R., Korzenevsky B.B. Biological peculiarities and structure of plants of Zingeria biebersteiniana (Claus) P. Smirn. (Poaceae). Ukr. Botan. Journ. 2010. Vol. 67 (5). P. 650–654. [in Russian]
Cremonini R. Low chromosome number angiosperm. Caryologia. 2005. Vol. 58 (4). P. 403–409.
Rodionov A.V., Kim E.S., Punina E.O., Machs E.M., Tyupa N.B., Nosov N.N. Evolution of chromosome numbers in the tribes Aveneae and Poeae inferred from the comparative analysis of the internal transcribed spacers ITS1 and ITS2 of nuclear 45S rRNA genes. Bot. Zhurn. 2007. Vol. 92 (1). P. 57–71. [in Russian]
Levenko B.A., Yurkova G.N., Kunakh V.A., Zosimovich V.P. Malokhromosomnyy zlak Zingeria – novyy model'nyy ob"yekt dlya kul'tury kletok i tkaney rasteniy. Doklady AN SSSR. 1976. Vol. 228 (1). P. 209–210. [in Russian]
Kunakh V.A. Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos, 2005. 724 p. [in Ukrainian]
Нussain A., Qarshi I.A., Hummera N., Ullah I. Plant Tissue Culture: Current Status and Opportunities. In: Recent Advances in Plant in vitro Culture. 2012. doi: 10.5772/50568.
da Silva A.L.L., Horbach M.A., Brondani G.E., Walter J.M., Dos Santos L.F., Habibi P., Araújo J.F., Kielse P. Applications of micropropagation in plant biotechnology. In book: Micropropagation: Methods and Effects. Publisher: Nova Science Publishers, Inc. 2019. P. 1–24.
Petrova T.F., Kulinich A.V., Abramova V.M. In vivo and in vitro cultivation of Zingeria biebersteiniana 2n equals 4 as a model genetic object. Doklady Akademii Nauk SSSR. 1991. 319 (4). P. 989–991. [in Russian]
Khromov A.V., Cherednichenko M.Yu. In vitro culture of Zingeria biebersteiniana (Claus) P. Smirn as a method of conservation and expanding its biodiversity. IOP Conf. Series: Earth and Environmental Science. 2022. 981. 042070. doi: 10.1088/1755-1315/981/4/042070.
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962. Vol. 15 (13). P. 473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x.
Gamborg O.L., Eveleigh D.E. Culture methods and detection of glucanases in cultures of wheat and barley. Can. J. Biochem. 1968. Vol. 46 (5). P. 417–421. doi: 10.1139/o68-063.
Lakin G.F. Biometrics: Textbook for biological specialties of universities. Moscow : Higher school, 1980. 293 p. [in Russian]
Saja J.Sh. In vitro study of the callus induction of two varieties of wheat seeds by plant growth regulators. J. Biochem. Cell. Arch. 2018. Vol. 18 (2). P. 2067–2071. doi: 10.3923/aj.2018.67.71.
