Agrobacterium-mediated transformation – method of genetic modification of Triticum aestivum L. plants
Abstract
Aim. Investigate the effect of Agrobacterium-mediated transformation in planta on seed tying and the frequency of transformation in winter wheat (Triticum aestivum L.). To analyze changes in the level of free L-proline (Pro) in transformed and control seedlings under normal / stress conditions and productivity indicators of biotechnological plants (T1) under normal growing conditions. Methods. Agrobacterium-mediated transformation in planta; PCR analysis, DNA electrophoresis; determination of seed tying frequency and transformation, Pro content, yield structure indicators. Results. Obtained transgenic wheat plants. The level of Pro in the tested variants under normal / stress conditions and indicators of T1 productivity of plants and their initial form under optimal water supply were studied. Conclusions. The susceptibility of the studied wheat genotypes to agrobacterial infection is shown. The frequency of seed tying after genetic transformation was 12.7 % and 5.4 % for plants of UK 106/19 and UK 171/19h, respectively. Transgenic seedlings had elevated levels of Pro. Complete incorporation of the vector construct was identified in 14 and 11 variants of genotypes UK 161/19 and UK 171/19h, respectively. Control and T1 biotechnological plants under normal growing conditions had similar yields.
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