Single Nucleotide Polymorphism of the chickpea gene encoding acetohydroxyacid synthase: molecular-genetical analysis

  • N. E. Volkova Cotecna Ukraine Limited, Ukraine, 65114, Odessa, Lustdorfska doroga str., 140a; Odessa State Agricultural Research Station of the National Academy of Agrarian Sciences of Ukraine, Ukraine, 67667, Odessa region, Bilyaiv district, Khlibodarske, Lighthouse doroga str., 24 https://orcid.org/0000-0002-9333-4872
  • H. Slishchuk Cotecna Ukraine Limited, Ukraine, 65114, Odessa, Lustdorfska doroga str., 140a https://orcid.org/0000-0003-4245-8557
  • V. Sichkar Odessa State Agricultural Research Station of the National Academy of Agrarian Sciences of Ukraine, Ukraine, 67667, Odessa region, Bilyaiv district, Khlibodarske, Lighthouse doroga str., 24
  • O. Zakharova Cotecna Ukraine Limited, Ukraine, 65114, Odessa, Lustdorfska doroga str., 140a https://orcid.org/0000-0003-1828-9044
DOI: https://doi.org/10.7124/FEEO.v30.1454
Keywords: chickpea, acetohydroxyacid synthase gene, single nucleotide polymorphism, herbicide resistance

Abstract

Aim. Chickpea AHAS gene molecular-marker analysis. To achieve this aim following tasks were set: chickpea AHAS gene SNP markers research and development, chickpea varieties and samples genotyping by the markers. Methods. CTAB method of DNA isolation and purification, real-time polymerase chain reaction. Results. Chickpea AHAS1 gene KASP gene specificity was found: there were no DNA amplification with lens and maize samples observed. Three Ukrainian chickpea varieties and 28 ICRISAT collection samples KASP genotyping detected only wild-type C-allele. Authors own SNP markers 58 chickpea samples genotyping showed that AHAS1 gene within samples was homozygous, no mutant T allele was detected. Conclusions. 31 chickpea samples contained wild-type C-allele by KASP genotyping, which is associated with imidazoline herbicide susceptibility. 58 chickpea samples contained C-allele by authors own SNP markers genotyping. Mutant T-allele that is associated with tolerance absence indicates researched samples imidazole herbicide tolerance low level.

References

Herbicide Resistance Action Committee. HRACMode of Action Classification 2020 Map. 2020. https://hracglobal.com/tools/hrac-modeof-action-classification-2020-map.

Garcia M., Nouwens A., Lonhienne T. Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families. Proc. Natl. Acad. Sci. USA. 2017. Vol. 114 (7). E1091-E1100. doi: 10.1073/pnas.1616142114.

Guttieri M., Eberlein C., Mallory-Smith C., Thill D., Hoffman D. DNA sequence variation in Domain A of the acetolactate synthase genes of herbicide-resistant and -susceptible weed biotypes. 1992. Weed Sci. Vol. 40. P. 670–676. http://www.jstor.org/stable/4045263.

Tranel P., Wright T. Resistance of weeds to ALS inhibiting herbicides: what have we learned? 2002. Weed Sci. Vol. 50. P. 700–712.

Duke S. Biotechnology: herbicide-resistant crops. In Encyclopedia of Agriculture and Food Systems (Van Alfen N., ed), Elsevier, San Diego. 2014. P. 94–116.

Cakir O., Ucari C., Tarhan C. Nutritional and health benefits of legumes and their distinctive genomic properties. Food Sci. Technol. 2019. doi: 10.1590/fst.42117.

Thompson С., Taran В. Genetic characterization of the acetohydroxyacid synthase (AHAS) gene responsible for resistance to imidazolinone in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 2014. Vol. 127. P. 1583–1591. doi: 10.1007/s00122-014-2320-0

He C., Holme J., Anthony J. SNP genotyping: the KASP assay. Methods Mol. Biol. 2014. Vol. 1145. 12 p. doi: 10.1007/978-1-4939-0446-4_7.

Rogers, S., Bendich, A. Extraction of DNA from plant tissues. In: Gelvin S.B., Schilperoort R.A., Verma D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. 1989. doi: 10.1007/978-94-009-0951-9.

Teggi A. Identification and characterization of herbicide tolerant mutant lines using SNP marker(s) in Chickpea (Cicer arietinum L.). 2017. http://oar.icrisat.org/id/eprint/10264.

Slishchuk H., Volkova N., Zakharova O., Korchmaryоvа A. Bioinformatic analysis of chickpea acetohydroxyacid synthase gene. Factors of experimental evolution of organisms. 2019. Vol. 24. P. 345–349. [in Ukrainian]