Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

  • N. A. Kozub
  • I.O. Sozinov
  • H.Ya. Bidnyk
  • N.O. Demianova
  • O.I. Sozinova
  • L.A. Yanse
  • A.V. Karelov
  • Ya.B. Blume
DOI: https://doi.org/10.7124/FEEO.v29.1412

Abstract

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.
Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.

References

Shewry P.R., Hey S.J. The contribution of wheat to human diet and health. Food and Energy Security. 2015. Vol. 4 (3). P. 178–202 doi: 10.1002/fes3.64.

Payne P.I. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annual Review of Plant Physiology. 1987. Vol. 38. P. 141–153.

Wrigley C.W., Asenstorfer R., Batey I.L., Cornish G.B., Day L., Mares D., Mrva K. The biochemical and molecular basis of wheat quality. Chapter 21. In: Carver B.F. (Ed.), Wheat: Science and Trade, Oxford. UK: Wiley-Blackwell, 2009. P. 495–520.

Payne P.I., Nightingale M.A., Krattiger A.F., Holt L.M. The relationship between the HMW glutenin subunit composition and the breadmaking quality of British-grown wheat varieties. J. Sci. Food Agric. 1987. Vol. 40. P. 51–65.

Kumar A., Kapoor P., Chunduri V., Sharma S., Garg M. Potential of Aegilops sp. for improvement of grain processing and nutritional quality in wheat (Triticum aestivum). Front Plant Sci. 2019. Vol. 10. e.308. doi: 10.3389/fpls.2019.00308.

Zhou J.P., Yao C.H., Yang E.N., Yin M.Q., Liu C., Ren Z.L. Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits. Genetics and Molecular Research. 2014. Vol. 13(1). P. 660–669. doi: 10.4238/2014.January.28.11.

Zhou J.P., Cheng Y., Zang L.L. Yang E.N., Liu C., Zheng X.L., Deng K.J., Zhu Y.Q., Zhang Y. Characterization of a new wheat-Aegilops biuncialis 1Mb(1B) substitution line with good quality-associated HMW glutenin subunit. Cereal Res. Commun. 2016. Vol. 44. P. 198–205. doi: 10.1556/0806.43.2015.048.

Wang S., Yu Z., Cao M., Shen X., Li N., Li X., Ma W., Weißgerber H., Zeller F., Hsam S., Yan Y. Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality. PLoS One. 2013. Vol. 8 (4). e58947. doi: 10.1371/journal.pone.0058947.

Garg M., Kumar R., Singh R.P., Tsujimoto H. Development of an Aegilops longissima substitution line with improved bread-making quality. J. Cereal Sci. 2014. Vol. 60 (2). P. 389–396. doi: 10.1016/j.jcs.2014.05.006.

Kozub N.O., Sozinov I.O., Bidnyk H.Ya., Demianova N.O., Sozinova O.I., Karelov A.V., Pylypenko L.A., Blume Ya.B., Sozinov O.O. Development and studying of Triticum aestivum L. material with introgressions from Aegilops biuncialis Vis. Factors of Experimental Evolution of Organisms. 2018. Vol. 23. P. 297–301. doi: 10.7124/FEEO.v23.1031

Kozub N.A., Sozinov I.A., Sobko T.A., Kolyuchii V.T., Kuptsov S.V., Sozinov A.A., Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine. Cytology and Genetics. 2009. Vol. 43 (1). P. 55–62. doi: 10.3103/S0095452717020050.

Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. Vol. 227, No. 5259. P. 680–685. doi: 10.1038/227680a0

Rybalka O.I., Chervonis M.V., Toporash I.G., Surzhenko I.O., Bodelan O.L., Shcherbyna Z.V. Scinetific substantiation of development of new methods for assessment of bread-making quality of wheat flour. Khranieniye i Pererabotka Zerna. 2006. No. 1 (79). P. 43–48. [in Ukrainian]

Lukaszewski A.J. Behavior of centromeres in univalents and centric misdivision in wheat. Cytogenet. Genome Res. 2010. Vol. 129. P. 97–109. doi: 10.1159/000314108.

Garg M., Tsujimoto H., Gupta R.K., Kumar A., Kaur N., Kumar R., Chunduri V., Sharma N.K., Chawla M., Sharma S., Mundey J.K.. Chromosome specific substitution lines of Aegilops geniculata alter parameters of bread making quality of wheat. PLoS One. 2016. Vol. 11 (10). e0162350. doi: 10.1371/journal.pone.0162350.

Rakszegi M., Molnár I., Lovegrove A., Darkó É., Farkas A., Láng L., Bedö Z., Doležel J., Molnár-Láng M., Shewry P. Addition of Aegilops U and M chromosomes affects protein and dietary fiber content of wholemeal wheat flour. Frotniers in Plant Science. 2017. Vol. 8, Article 1529. doi: 10.3389/fpls.2017.01529.

Rybalka O.I. Wheat quality and its improvement. Kyiv: Logos, 2011. 496 p. [in Ukrainian]