Research of protein complex of F3 hybrids chickpea seed

  • O. O. Molodchenkova
  • G. D. Lavrova
  • T. V. Kartuzova
  • L. Ya. Bezkrovna
  • O. B. Lykhota
  • O. V. Ryshchakova
  • Yu. A. Levitsky
DOI: https://doi.org/10.7124/FEEO.v27.1334

Abstract

Aim. The aim of the study was to investigate the peculiarities of the protein complex of seeds of F3 hybrid populations and their parental forms of chickpea (Cicer arietinum L.) of Ukrainian and foreign breeding. Меthods. Research methods are  standard methodologies of  plant biochemical analysis  and methods developed  in the laboratory (Kjeldahl method, spectrophotometric methods, protein electrophoresis). Results. The peculiarities of protein content, basic fractions of globulins – legumin and vicilin, activity of trypsin inhibitor, lectins, lipoxygenase in the seeds of F3 hybrid populations and their parent forms of chickpea have been established. It was shown using electrophoretic and densitometric analyses that F3 hybrid populations of chickpea  were non identifical  in level and character of  globulin component composition variability.  The clear differences are observed both in the intensity of the staining and the displacement of  protein components of legumin and vicilin which are identical in mobility,  presence and absence of components that are typical for  particular hybrid combination. Conclusions. Obtained results can be used to selection of chickpea genotypes of food direction.

Keywords: сhickpea, protein,  vicilin, legumin, trypsin inhibitor.

References

Messina M.J. Legumes and soybeans: overview of their nutritional profiles and health effects. The American Journal of Clinical Nutrition. 1999. Vol. 70. P. 439–450. doi: 10.1093/ajcn/70.3.439s

Yaklich R.W. β-Congycinin and glycinin in high protein soybean. G. Agric. Food.Chem. 2000. Vol. 49. P. 729–735. doi: 10.1021/jf001110s

Dadon S.B.E., Pascual C.Y., Eshel D., Teper-Bamnolker P., Ibanez M.D.P., Reifen R. Vicilin and the basic of legumin are putative chickpea allergens. Food chemistry. 2013. Vol. 138. P. 13–18. doi: 10.1016/j.foodchem.2012.10.031

Khokhar S., Apenten R.K.O. Antinutritional factors in food legumes and effects of processing. In book: The role of food, agriculture, forestry and fisheries in human nutrition. Edition: 1, Chapter: Publisher: Encyclopedia of Life Support Systems (EOLSS). Publishers Co Ltd., Oxford, UK, P. 82–116.

Lucik М.F., Pаnasyuk Е.N., Lucik А.D. Lectins. Lviv: Vusha shkola, 1981. 150 p. [in Russian]

Budnickaya Е.V. Research of lipoxygenase activity of feeder greens by methods of carotin oxidation. Biochemistry. 1955. Vol. 20 (5). P. 615–621. [in Russian]

Levitsky A.P. Methods of determination of trypsin inhibitors. Biochemical methods of breeding material research. Odessa: VSGI, 1979. Vol. 15. P. 68–72. [in Russian]

Аdamovskaya V.G., Моlodchenkova О.О., Sіchкаr V.І., Ciselskaya L.Y., Sagaydak Т.V. Method of soybean selection: Pat. on utility model 42181 Ukraine. Declare 25.06.2009, publish. 15.11.2009. Bulletin No. 1. [in Ukrainian]

Аdamovskaya V.G., Моlodchenkova О.О., Каrtuzova Т.V., Sіchkar V.І., Lavrova G.D. Method of soybean genotypes selection of food direction: Pat. on utility model 107671 42181 Ukraine. Declare 24.06.2016, publish 15.11.2016. Bulletin No. 1. [in Ukrainian]

Adamovskaya V.G., Molodchenkova O.O., Sichkar V.I., Lavrova G.D., Kartuzova T.V., Bezkrovnaya L.Ya. The content of 7S and 11S globulins and their relationship with the protein content of seeds of soybean hybrid populations. Collected scientific articles of PBGI-NCSCI. 2014. No. 23 (63). P. 37–42. [in Russian]

Change Y., Alli I., Molina A., Konishi Y., Boye J. Isolation and characterization of chickpea (Cicer arietinum L.) seed protein fraction. Food and Bioprocess Technilogy. 2012. Vol. 5. P. 618–625. doi: 10.1007/s11947-009-0303-y