Identification of leaf rust resistance gene Lr34/Yr18/Sr57/Pm38/Bdv1 in soft wheat varieties
Abstract
Aim. Analysis of the allelic composition of the csLV34 locus and the microsatellite locus Xgwm295 for the detection of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene, which determines the resistance of modern wheat varieties of domestic breeding of diseases: leaf rust and powdery mildew. Methods. Molecular genetic (DNA isolation, polymerase chain reaction, electrophoresis of amplification products) and phytopathological methods. Results. A study of soft wheat varieties of Ukrainian breeding was carried out. Two csLV34а and csLV34b alleles were identified that correlate with the absence and presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene. 17.6 % of the cultivars tested were found to have the csLV34b allele, which is associated with the presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene and resistance to brown rust. Conclusions. PCR revealed six varieties of soft wheat that contain the allele csLV34b, which is associated with the presence of the gene Lr34/Yr18/Sr57/Pm38/Bdv1. The information obtained can be used in breeding programs to create new varieties resistant to disease.
Keywords: leaf rust, powdery mildew, soft wheat, polymerase chain reaction, varieties, stability.
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