Application of mycorrhizal fungus Tuber melanosporum to stimulate the growth and development of soybean and spring barley
Abstract
Aim. To evaluate the effect of soybean and spring barley seeds treatment with haploid cells of fungus Tuber melanosporum IMB F- 100106 on the growth and development of soybean and spring barley. Methods. The study was conducted in field conditions on the experimental field of the Department of Agroecology and Biosafety IAP NANU. The level of development of the fungus was evaluated visually and by counting ectomycorrhizal threads. Morphometric and biochemical methods were used to characterize the structure of the crop. Results. The treatment of soybean and spring barley seeds with T. melanosporum promotes productive growth and increases the yield of crops. Conclusions. Plant inoculation with T. melanosporum promotes productive growth and increases the yield of soybean and barley, promotes the possibility of creating new technologies of organic and regenerative agriculture and allows preserving of the genetic stability of this fungus.
Keywords: mycorrhiza, Tuber melanosporum, crop yields, genetic stability.
References
Fassi B., Fontana A. Sintesi mycorrizica tra Pinus strobus e Tuber maculatum – I. Mycorrize e sviluppo dei semenzali nel sec-ondo anno. Allionia. 1967. Vol. 13. P. 177–186.
Gioacchini A.M., Menotta M., Bertini L., Rossi I., Zeppa S., Zambonelli A., Piccoli G., Stocchi V. Solid-phase microextrac-tion gas chromatography/mass spectrometry: a new method for species identification of truffles. Rapid Communications in Mass Spectrometry. 2005. Vol. 19. P. 2365–2370.
Nardinia A., Salleo S., Tyree M.T., Vertovec M. Influence of the ectomycorrhizas formed by Tuber melanosporum Vitt. on hydraulic conductance and water relations of Quercus ilex L. seedlings. Annals of Forest Science. 2000. Vol. 57. P. 305–312. doi: 10.1051/forest:2000121.
Olivera A., Bonet J.A., Palacio L., Liu B., Colinas C. Weed control modifies Tuber melanosporum mycelial expansion in young oak plantations. Annals of Forest Science. 2014. Vol. 71. P. 495–504. doi: 10.1007/s13595-014-0360-x.
Wenkart S., Roth-Bejerano N., Mills D., Kagan-Zur V., Mycorrhizal association between Tuber melanosporum mycelia and transformed roots of Cistus incanus. Plant Cell Rep. 2001. Vol. 20. P. 369–373.
Pinkas Y., Maimon M., Shabi E., Elisha S., Shmulewich Y., Freeman S. Inoculation, isolation and identification of Tuber melanosporum from old and new oak hosts in Israel. Mycol. Res. 2000. Vol. 104. P. 472–477.
Tacon F., Rubini A., Murat C., Riccioni C., Robin C., Belfiori B., Zeller B., De la Varga H., Akroume E., Deveau A., Martin F., Paolocci F. Certainties and uncertainties about the life cycle of the Pйrigord black truffle (Tuber melanosporum Vittad). Annals of Forest Science. 2016. Vol. 73. P.105–117. doi: 10.1007/s13595-015-0461-1.
Sally E., Smith F.A.A, David Read F.R.S. Mycorrhizas in agriculture, horticulture and forestry, in Mycorrhizal Symbiosis (Third Edition). 2008. 767 p.
Solti Б., Tamaskу1G., Lenk S., Barуcsi A., Bratek Z. Detection of the vitalization effect of Tuber mycorrhiza on sessile oak by the recently-innovated FMM chlorophyll fluorometer. Acta Biologica Szegediensis. 2011. Vol. 55. P. 147–149.
Liu Q.N., Liu R.S., Wang Y.H., Mi Z.Y., Li D.S., Zhong J.J., Tang Y.J. Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides. Bioresour Technol. 2009. 100 (14). P. 3644–3649.
Comandini O., Contu M., Rinaldi A.C. An overview of Cistus ectomycorrhizal fungi. Mycorrhiza . 2006. Vol. 16. P. 381–395. doi: 10.1007/s00572-006-0047-8.
Selosse M.A., Martin F., Bouchard D., le Tacon F. Structure and dynamics of experimentally introduced and naturally occur-ring Laccaria sp. Discrete genotypes in a Douglas fir plantation. Appl. Environ. Microb. 1999. Vol. 65. P. 2006–2014.
Belfiori B., Riccioni C., Tempesta S., Pasqualetti M., Paolocci F., Rubini A. Comparison of ectomycorrhizal communities in natural and cultivated Tuber melanosporum truffle grounds. FEMS Microbiol Ecol. 2012. Vol. 81. P. 547–561. doi: 10.1111/j.1574-6941.2012.01379.
Liu B., Fischer C.R., Bonet J.A., Castaсo C., Colinas C. Shifts in soil fungal communities in Tuber melanosporum plantations over a 20-year transition from agriculture fields to oak woodlands. Forest Systems. 2016. Vol. 25. eSC05. doi: 10.5424/fs/2016251-08353.