Genetic diversity of spring common wheat varieties at storage protein loci

  • T. O. Sobko
  • L. V. Sirant
  • G. M. Lisova
DOI: https://doi.org/10.7124/FEEO.v23.1037

Abstract

Aim. The aim of this study was to investigate genetic diversity of storage protein loci in spring common wheat varieties licensed for growing in Ukraine in 2017 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the gliadin loci Gli-1 and the high-molecular-weight glutenin subunit loci Glu-1. Results. Genotypes at the gliadin loci Gli A1, Gli B1, Gli D1 and glutenin loci Glu A1, Glu B1, Glu D1 were identified in 31 spring common wheat. The wheat-rye translocation AL/1RS was identified in two varieties Etyud and Struna mironivsyka. Differences in frequencies of alleles at storage protein loci were revealed. Conclusions. The high level of allelic variation was observed at the Gli-1 loci in spring common wheat varieties. Predominant alleles (one or two per locus) were revealed: Gli A1f, Gli A1а, Gli B1e, Gli D1b, Gli D1і, Gli D1f , Glu А1а, Glu Bс , Glu D1d, Glu D1a.

Keywords: Triticum aestivum L., spring wheat, storage protein.

References

Catalogue of gene symbols. Gene catalogue, 2013. URL: htpp://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jspMacGene (last accessed: 16.03.2018).

Sobko T.A., Poperelya F.A. The frequency of alleles of gliadin-coding loci in different cultivars of winter common wheat. Visnyk s.-h. nauky. 1986. Vol. 5. P. 84–87 [in Ukrainian].

Metakovsky E.V. Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. J.Genet. and Breed. 1991. Vol. 45. P. 325–344.

Payne P.I., Lawrence G.J. Catalogue of alleles for the complex geneloci, Glu-A1, Glu-B1, and Glu-D1 which code for high-mole- cular-weight subunits of glutenin in hexaploid wheat. Cereal Res.Commun. 1983. Vol. 11, No. 1. P. 29–34.

Chernakov K.M., Metakovsky E.V. Diversity of Gliadin-Coding Locus Allelic Variants and Evaluation of Genetic Similarity f Common Wheat Varieties from Different Breeding Centers. Genetika, 1994. Vol. 30. P. 509–517 [in Russian].

Rabinovich S.V., Panchenko I.A., Parchomenko R.G., Usova Z.U. High-molecular weight glutenin subunit composition of spring common wheats grown in the Ukraine and the Russian Federation in 1995-97 and their connection with pedigrees. Ann Wheat Newslet. 1997. Vol. 44. P. 236–251.

Sobko T.A., Sozinov A.A. Analysis of the genotypic structure of common wheat cultivars licensed for growing in Ukrainne using genetics markers. Tsitologiya i genetika. 1999. Vol. 33, No. 5. P. 30–41 [in Russian].

Kozub N.A., Sozinov I.A., Sobko T.A., Kolyuchii V.T., Kuptsov S.V., Sozinov A.A. Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine. Cytol. Genet. 2009. Vol. 43, No. 1. P. 55–62.

Kozub N.A., Sozinov I.A., Karelov A.V., Blume Ya.B., Sozinov A.A. Diversity of Ukrainian winter common wheat varieties with respect tostorage protein loci and molecular markers for disease resistance genes. Tsitologiya i genetika. 2017. Vol. 51, No. 2. P. 59–73 [in Ukrainian]

Poperelya F.A. Three main genetic systems of the grain quality in winter common wheat. Realization of potential possibilities of varieties and hybrids of the Selection-Genetic Institute in conditions of Ukraine. Zbirnyk naukovykh prats SHI. 1996. P. 117–132 [in Ukrainian].

State register of plant varieties suitable for dissemination in Ukraine in 2017. URL: http://www.minagro.gov.ua/rating/files/r2.pdf (Last accessed: 6.03.2018).

Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. Vol. 227. P. 680–685.

Metakovsky E.V., Chernakov V.M., Upelniek V.P.,Redaelli R., Dardevet M., Branlard G., Pogna N.E. Recombination mapping of minor w-gliadincoding loci on chromosome 1A of common wheat: A revision. J. Genet. Breed. 1996. Vol. 50. P. 277–286.

Rabinovich, S.V. Importance of Wheat-Rye Translocations for Breeding Modern Cultivars of Triticum aestivum L. Euphytica. 1998. Vol. 100. P. 323–340.

Vlasenko V.A., Molotsky M.Ya., Sobko T.O., Kozub N.O., Solonа V.Y. Selective value of wheat-rye translocation of 1AL/1RS for the breeding of spring common wheat varieties. Visnykh Bilotserkovskoho derzhavnoho ahrarnoho universytetu. Zbirnyk naukovykh prats. 2006. Vol. 35. P. 30–37 [in Ukrainian].

Novosel’skaya-Dragovich A.Yu., Fisenko A.V., Puckhal’skii V.A. Genetic differentiation of common wheat cultivars using multiple alleles of gliadin-coding loci. Genetika. 2013. Vol. 49, No. 5. P. 569–579 [in Russian]. doi: 10.7868/S0016675813020082.

Sobko T.O., Sozinov O.O. Genetic analysis of the gene pool of spring common wheat varieties of Ukraine the storage proteins loci. Genetic resources for adaptive plant growing: mobilization, inventory, preservation, using: tezy dopovidei mizhn. nauk. konf. (Obroshyno, 29 chervnia – 1 lypnia 2005 r.). Obroshyno, 2005. P. 253–254. [in Ukrainian].