Chickpea genotypes characteristics on resistance to fusarium Fusarium oxysporum f. sp. ciceris

  • V. A. Chekalov
  • N. E. Volkova
DOI: https://doi.org/10.7124/FEEO.v23.1008

Abstract

Aim. Molecular-genetic analysis of the chickpea genotypes for foc0, foc3, foc4 resistance genes to Fusarium oxysporum f. sp ciceris. Methods. Extraction and purification of DNA, spectrophotometry, polymerase chain reaction, electrophoresis in polyacrylamide gels. Results. 35 chickpea lines and varieties of Ukrainian and foreign breeding characterized according to genotyping on foc0, foc3, foc4 genes of resistance to Fusarium oxysporum f. sp ciceris by the microsatellite markers TA59, TR19 and TR59. Fragments of the expected size for all markers were obtained for samples, for which the resistance level was fixed to certain races. Match between data on the presence of a amplification fragment of a certain size and resistance level among other samples is not found. Conclusions. For 35 chickpea varieties and lines the allele state of foc0, foc3, foc4 genes of resistance to the F. oxysporum f. sp ciceris races 0, 3, 4 is established. The variety ‘Pam’yat’ is recommended as a control of resistance to F. oxysporum f. sp ciceris races 0, 3, 4 in the southern Ukraine conditions.

Keywords: chickpea, genes, molecular markers, fusarios, resistance.

References

Sharma K.D., Muehlbauer F.J. Fusarium wilt of chickpea: Physiological specialization, genetics of resistance and resistance gene tagging. Euphytica. 2007. Vol. 157. P. 1–14. doi: 0.1007/s10681-007-9401-y

Winter P., Pfaff T., Udupa S. M., Huttel B., Sharma P. C., Sahi S., Kahl G. Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol. General Genet. 1999. Vol. 262. P. 90–101.

Sharma K.D., Chen W., Muehlbauer F. J. Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Disease. 2005. Vol. 89. P. 385–390. doi: 10.1094/PD-89-0385

Padaliya R.V., Suthar K.P., Diwakar S., Mahatma M.K., Patil V.R. Marker assisted characterization of chickpea genotypes for wilt resistance. African J. Biotech. 2013. Vol. 50. P. 6907–6912. doi: 10.5897/AJB2013.13039