Визначення алельного стану генів Glu-1 в гібридних сім’ях, носіїв гена Gpc-B1 від Triticum turgidum ssp. dicoccoides

  • С. Ю. Похилько
  • А. І. Степаненко
  • О. М. Дуган
  • Б. В. Моргун

Анотація

Aim. The aim of our study was to analyze the hybrid families of generation F5 carrying gene Gpc-B1 of Triticum turgidum ssp. dicoccoides for the allelic state of Glu-1, to calculate 1000 kernel weight and to select families with the best set of alleles and performance. Methods. Polymerase chain reaction for molecular genetic analysis and calculation of the 1,000-kernel weight for wheat grain yields were used. Results. Determination of the three loci Glu-A1, Glu-B1, Glu-D1 shows that among 44 hybrid families it is possible to identify 16 most promising families that have the most valuable allelic variants. Furthemore, given the analysis of the 1,000-kernel weight, two families (#36 and 40) having the highest values can be selected among those 16 families. Conclusions. The results of study enable a comprehensive approach to the selection of progeny with the best genetic base, which will be used in breeding of soft winter wheat later on.

Keywords: Triticum aestivum L., gene Gpc-B1, Glu-1 loci, PCR, molecular markers.