Визначення алельного стану генів Glu-1 в гібридних сім’ях, носіїв гена Gpc-B1 від Triticum turgidum ssp. dicoccoides

С. Ю. Похилько, А. І. Степаненко, О. М. Дуган, Б. В. Моргун

Анотація


Aim. The aim of our study was to analyze the hybrid families of generation F5 carrying gene Gpc-B1 of Triticum turgidum ssp. dicoccoides for the allelic state of Glu-1, to calculate 1000 kernel weight and to select families with the best set of alleles and performance. Methods. Polymerase chain reaction for molecular genetic analysis and calculation of the 1,000-kernel weight for wheat grain yields were used. Results. Determination of the three loci Glu-A1, Glu-B1, Glu-D1 shows that among 44 hybrid families it is possible to identify 16 most promising families that have the most valuable allelic variants. Furthemore, given the analysis of the 1,000-kernel weight, two families (#36 and 40) having the highest values can be selected among those 16 families. Conclusions. The results of study enable a comprehensive approach to the selection of progeny with the best genetic base, which will be used in breeding of soft winter wheat later on.

Keywords: Triticum aestivum L., gene Gpc-B1, Glu-1 loci, PCR, molecular markers.


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