Expression analysis of alpha-tubulin genes during cold acclimation in winter wheat Demetra

  • D. D. Bui
  • A. Ye. Demkovych
  • Ya. V. Pirko
  • Ya. B. Blume


Aim. Wheat (Triticum aestivum L.) display significant increase in freezing tolerance during a period of cold acclimation (CA). Functions and regulations unraveling of CA-associated genes can help in cold-resistant cultivars receiving using biotechnology methods. Cytoskeleton reorganization is an important element of the cold resistance mechanism associated with tubulin expression level alterations. Methods. Cold acclimation during 28 days was carried out. The expression level of 15 members of alpha-tubulin genes was measured using RT Real-time PCR with specific primers. Results. Similar patterns of expression alterations were observed within subfamilies. The highest levels of expression were recorded for the fourth subfamily members (Tuba_4-1, 4-2, 4-3) and Tuba_2-3 gene. These 4 alpha-tubulin genes may be involved in the vital functions maintenance during the first days of the low temperature influence. Conclusions. It is likely that four of the fifteen genes are related to cold tolerance mechanisms since they have significantly higher expression levels during cold acclimation.

Keywords: Triticum aestivum, winter wheat, tubulin, cold acclimation.


Christov N.K., Imai R., Blume Y.B. Differential expression of two winter wheat alpha-tubulin genes during cold acclimation. Cell Biol. Int. 2008. V. 32. P. 574-578. doi: 10.1016/j.cellbi.2007.11.014

Gray G.R. Cold acclimation and freezing tolerance: a complex interaction of light and temperature. Plant Physiol. 1997. V. 114. P. 467-474. doi: 10.1104/pp.114.2.467

Tardif G., Kane N.A., Adam H., Labrie L., Major G., Gulick P., Sarhan F., Lalibert J.F. Interaction network of proteins associated with abiotic stress response and development in wheat. Plant Mol. Biol. 2007. V. 63. P. 703-718. doi: 10.1007/s11103-006-9119-6

Abdrakhamanova A., Wang Q.Y., Khokhlova L., Nick P. Is microtubule disassembly a trigger for cold acclimation? PlantCellPhysiol. 2003. V. 44. P. 676-686. doi: 10.1093/pcp/pcg097

Jian L.C., Sun L.H., Liu Z.P. Studies on microtubule cold stability in relation to plant cold hardiness. Acta Bot. Sinica. 1989. V. 31. P. 737-741.

Kerr G.P., Carter J.V. Tubulin isotypes in rye roots are altered during cold acclimation. Plant Physiol. 1990. V. 93. P. 83-88. doi: 10.1104/pp.93.1.83

Ridha Farajalla M., Gulick P.J. The alpha-tubulin gene family in wheat (Triticum aestivum L.) and differential gene expression during cold acclimation. Genome. 2007. V. 50. P. 502-519. doi: 10.1139/G07-027

Maibam P., Nawkar G.M., Park J.H., Sahi V.P., Lee S.Y., Kang C.H. The influence of light quality, circadian rhythm, and photoperiod on the CBF-mediated freezing tolerance. Int. J. Mol. Sci. 2013. V. 14. P. 11527-11543. doi: 10.3390/ijms140611527

Xiong L., Schumaker K.S., Zhu J.K. Cell signaling during cold, drought, and salt stress. Plant Cell. 2002. V. 14. P. 165-183. doi: 10.1105/tpc.000596

Buy D.D., Pirko Ya.V., Blume Ya.B. Expression of winter and spring wheat alpha-tubulin genes during cold acclimation. Faktory eksperymental'noi evoliutsii. 2015. V. 17. P. 27-30. [in Ukrainian]