Рослини Deschampsia antarctica E. Desv. з різним числом хромосом в умовах вирощування in vitro. Довжина листків та вміст флавоноїдів у культурі in vitro та в природі

О. О. Пороннік, І. Ю. Парнікоза, Н. Ю. Мірюта, Г. Ю. Мирюта, В. П. Грахов, Д. О. Навроцька, В. А. Кунах

Анотація


Aim. The aim of this work is to research two adaptability indices (leaf length and flavonoid content and spectrum) in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. in standardized cultivation condition in vitro. Then these adaptability indices would compare with the same for native populations. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves, HPLC method for research of flavonoid spectrum in plant leaves. Results. Heterogeneity of leaf length and flavonoid content has been shown. Both parameters difference have been detected in comparison with native populations. Flavonoid spectrum has no difference in comparison with native populations. Conclusions. Deschampsia antarctica genotypes under standardized cultivation condition in vitro have had longer leaves than plants in nature populations. Length of the leaves was varied depending on karyotype of corresponding genotype. Cultivated plants have had flavonoid spectrum similar to the same plants in nature. This fact allows to assume plants in vitro possible producent of secondary metabolites.

Keywords: Deschampsia antarctica, leaf length, flavonoids, chromosome number.


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