Жирно-кислотний склад калюсних культур з різних типів експлантів рослин амаранту

В. П. Жук, Л. О. Сахно, М. А. Хархота, С. В. Ісаєнков

Анотація


Aim. The aim of our study was selection of optimal conditions for the initiation of Amaranthus L. aseptic callus in vitro culture from different types of explants and estimation of fatty acid composition in these types of cell cultures. Methods. In vitro culture using leaf disks and internodes as explants. Inert gas chromatography. Results. The optimized Gamborg medium for callus induction was designed (sucrose 25 g/1L; 2.4-D 0.5 mg/L; NАА 1 mg/L). The кіnetin in concentration 0.5 mg/L for internodes and for leaf discs explants were added. The fatty acid profiles of calli cultures from the different types of plant explants were analyzed The highest level of Omega-3 fatty acid were detected in cell cultures from internodes and leaf discs of cultivar Helios. Conclusions. Our optimized protocol for Amaranthus callus initiation could be used for further studies of the synthesis and accumulation of biologically active metabolites in Amaranthus tissue culture. The fatty acid composition of calli cultures depend from explant type as well as from plant cultivar.

Keywords: Amaranthus, callus, in vitro culture, fatty acid composition


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