Гени контролю темпів розвитку як компоненти системи регуляції стійкості Triticum aestivum L. до біотичного стресу за умов in vitro
Aim. The aim of our work is to research the influence of exometabolites phytopathogens of genus Fusarium on callus culture isogenic lines for genes PPD (NILs) of winter soft wheat. Methods. In the work used standard biotechnological and mycological methods. The influence of exometabolites phytopathogens g. Fusarium investigated, adding CF mi-cromycetes to MS culture medium in a ratio of 1:20, using transplants callus culture of isogenic lines of wheat. Growth index analyzed density of callus tissue and size of callus cells determined. Results. Established that the CF of Fusarium oxysporum significantly slows grows reaction of callus cultures and morphological structure callus NILs is changes. It is shown that the impact exometabolites phytopathogens has the opposite effect on cytological parameters (number and length callus cells) in the isolines differing pace of development in conditions in vivo. The culture filtrate F. oxysporum has more toxicity compared to exometabolites F. moniliforme. Conclusions. It is supposed that the genetic system controlling the pace of development and photoperiodic sensitivity Triticum aestivum L. in conditions in vivo indirectly determines the formation of resistance to biotic stress conditions in vitro.
Keywords: Triticum aestivum L., Fusarium oxysporum, Fusarium moniliforme, PPD genes, NILs, callus culture, growth index, resistance to phytopathogens.