Поліморфізм мікросателітних локусів Globoderа pallida з популяцій різного походження
Aim. The comprehensive population studies were conducted to elucidate the genetic diversity and virulence of Globodera pallida. Methods. The population studies were combined with SSR-analysis of genetic diversity inferred from six microsatellite loci to characterize 10 populations of G. pallida of different origin. Results. A phylogenetic analysis based on six microsatellite loci genotyping data showed that the Ukrainian populations of Globodera pallida were almost identical to other Globodera pallida populations from Europe representing Pa2/3 virulence group. Limited genetic variability inferred from the microsatellite loci polymorphisms was observed between Globodera pallida populations distributed in Europe and Ukraine, accounting for 82.3–93.4 % (P<0.05) of the genetic variability within the populations studied. These results support a hypothesis that populations in Ukraine are the result of the continuing spread of Globodera pallida within Europe and not the result of additional introductions from South America. Conclusions. The panel of 6 microsatellite loci allowed to investigate the genetic variability of Globodera pallida populations of different geographical origin and selection on three sources of resistance. The results revealed that selected Globodera pallida populations were similar but not identical indicating that changes in allele and genotype frequency had taken place as a result of the selection regime, but these neutral markers were not yet applicable for virulence monitoring.
Keywords: potato cyst nematodes, microsatellite loci, phylogeneic relationship, virulence.