Heterozygos eletion of β-catenin in early cardiogenesis attenuated the heart growth and affected on canonical Wnt kinetics

  • O. L. Palchevska Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • A. A. Hazaeeva Karazin Kharkiv National University, Ukraine, 61022, Kharkiv, Svobody Sqr., 4
  • V. V. Balatskyi Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • T. P. Ruban Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • L. L. Matsevych Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • O. O. Piven Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
DOI: https://doi.org/10.7124/FEEO.v20.739

Abstract

Aim. In our present work, we have analyzed newborn and embryonic heart under the β-catenin haploinsufficiency. Methods. Beta-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. In such way we generate the β-catenin haploinsufficient new born (P1-2) and embryonic hearts (E12,5 an E14,5) With rtPCR using we analyze the canonical WNT signalling kinetics in embryonic and newborn hearts. Namely we have analyzed the level of TСF-4, Axin2, c-Fos and CyclinD2 genes expression. Beside of this we have studied the γ-catenin gene expression un-der normal and β-catenin haploinsufficient conditions. Results. Cardiac β-catenin knockout leads attenuated newborn heart growth and associated with γ-catenin expression up-regulation. Canonical Wnt signalling activated in later cardiogenesis (E12,5-14,5) in WT heart and downregulated in newborns. Conclusions. We have shown the importance of canonical Wnt during later cardiogenesis. Thus β-catenin haploinsufficiency leads to violation of WNT kinetic in latter embryos and attenuated the heart growth.
Keywords: heart development, cardiogenesis, WNT signalling, β-catenin, γ-catenin.

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