Гетерозиготна делеція гена β-катеніну у ранньому кардіогенезі спричиняє затримку росту серця і порушує кінетику канонічного Wnt сигналінгу

О. Л. Пальчевська, А. А. Хазєєва, В. В. Балацький, Т. П. Рубан, Л. Л. Мацевич, О. о. Півень

Анотація


Aim. In our present work, we have analyzed newborn and embryonic heart under the β-catenin haploinsufficiency. Methods. Beta-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. In such way we generate the β-catenin haploinsufficient new born (P1-2) and embryonic hearts (E12,5 an E14,5) With rtPCR using we analyze the canonical WNT signalling kinetics in embryonic and newborn hearts. Namely we have analyzed the level of TСF-4, Axin2, c-Fos and CyclinD2 genes expression. Beside of this we have studied the γ-catenin gene expression un-der normal and β-catenin haploinsufficient conditions. Results. Cardiac β-catenin knockout leads attenuated newborn heart growth and associated with γ-catenin expression up-regulation. Canonical Wnt signalling activated in later cardiogenesis (E12,5-14,5) in WT heart and downregulated in newborns. Conclusions. We have shown the importance of canonical Wnt during later cardiogenesis. Thus β-catenin haploinsufficiency leads to violation of WNT kinetic in latter embryos and attenuated the heart growth.
Keywords: heart development, cardiogenesis, WNT signalling, β-catenin, γ-catenin.


Повний текст:

PDF

Посилання

  • Поки немає зовнішніх посилань.