Регуляція експресії гена MGMT естрогеном у клітинах людини in vitro
Aim. The repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) plays a dual role. It protects cell against DNA’s alkylation and cell death. But in the case of cancer treatment it provides the cancer cells’ resistance to alkylating chemotherapy. So it is important to regulate MGMT activity to inhibit it in cancer cells and to activate in normal cells. To treat some type of cancers used combination of the chemotherapy and the hormone therapy. So, we tried to investigate the role of estrogen on the MGMT transcription. Methods. We performed western-blot and RT-qPCR to analyse the human MGMT expression on mRNA and protein level. Results. We observe MGMT upregulation on both mRNA and protein level at the 0.5–1 nM estrogen concentration relative to untreated control. Conclusions. Estrogen is one of the potential hormonal MGMT regulators.
Keywords: O6-methylguanine-DNA methyltransferase (MGMT), estrogen, steroid hormones.