Мутанти B. subtilis з інсерцією Alu-повтору людини характеризуються підвищеною чутливістю до цитостатичної дії лектинів
Aim. Unique B. subtilis mutants with altered cytokinesis that were supposed to be a result of human Alu-repeat insertions have been investigated to determine possible alterations of the genome, the sensitivity to bacteriostatic action of lectins and search for potential targets of such action. Methods. Rep-PCR genomic fingerprinting with use of PCR markers REP, ERIC and BOX was applied. Commercial lectins with different structures: PHA-Е, PHA-L SNA, and WGA (LECTINOTEST, Ukraine) were used. Search for lectin’s targets was performed by inhibitory analysis using diffuse agar test. Results. Alu-repeat insertions did not affect significantly the amplification profile of B. subtilis own noncoding sequences – REP, ERIC and BOX. Alu-integrant mutants demonstrated the increased sensitivity to the cytostatic effect of lectins. Inhibitory analysis showed that the possible targets of the lectins under study may be associated with cell surface or process of protein synthesis, depending on the lectin structure. Conclusions. The unique bacterial model is adequate for both the theoretical study of the mechanisms of lectin action and practical rapid tests of these biologically active substances cytostatic effects.
Keywords: Bacillus subtilis mutants, Alu-repeat insertion, PCR markers, lectins, inhibitory analysis.