Microsatellite instability in ICR mouse strain, predisposed malignant swellings

  • M. V. Kovalchuk Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine Ukraine, 03143, Kiev, Zabolotnogo str., 150
  • T. P. Gulko Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine Ukraine, 03143, Kiev, Zabolotnogo str., 150; State Institute “Institute of Genetic and Regenerative Medicine NAMS Ukraine” Ukraine, 04114, Kiev, Vyshgorodska, 67

Abstract

Aims. Microsatellite instability (MSI) is a form of genomic instability frequently detected in many types of tumors. However, the involvement of MSI in cancer development in the population ICR mouse strain has not been investigated. The aim of this work was to determine the reveal of loss of heterozygosity(LOH) and microsatellite instability (MSI) and to evaluate the possibility ofusing LOH and MSI as molecular markers for the detection ofgenomic alterations. Methods. Genomic DNA obtained from peripheral blood lymphocytes from each animals was subjected to PCR using specific primers. The PCR products were separated by 10% non-denaturingPAGE and analyzed for the presence of LOH and MSI. Results. The presence of these microsatellitealterations was related both to the carcerous phenotype and to the mouse strain. Conclusion. This study has demonstrated that microsatellite alterations occurin a part of ICR population butour data need further validation with a larger number of microsatellite loci and enhanced method for MSI identification.

Key words: microsatellite instability, loss of heterozygosity, tumorigenesis.