Development of alien-substitution and alien-addition Triticum aestivum / Aegilops mutica lines

  • T. S. Iefimenko National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2
  • M. Z. Antonyuk National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2
  • V. S. Martynenko National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2

Abstract

Aims. Development of T. aestivum / Ae. mutica introgressive lines and their assessment for powdery mildew resistance and morphological characters. Methods. Artificial hybridization, determination of chromosome numbers at mitosis metaphase, PCR, dot-blot hybridization of genome DNA. Results. AABBDT hybrid was sufficiently self-fertile; therefore, progeny derived from its self-pollination were used for the development of introgressive lines, according to chromosome mixing method. Avoidance of backcrossing to recurrent parent provided maximal retention of Aegilops chromosomes in progeny used for introgressive lines development. Conclusions. Derived introgressive lines are appropriate for the following identification of quantity and amount of alien introgressions and determination of their homoeological identity.

Key words: wheat introgressive lines, Ae. mutica, powdery mildew, frost resistance.