Simulation of liver cirrhosis in rats Wistar different ages

  • T. P. Gulko Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine Ukraine, 03143, Kiev, Zabolotnogo str., 150; State Institute “Institute of Genetic and Regenerative Medicine NAMS Ukraine” Ukraine, 04114, Kiev, Vyshgorodska, 67
  • M. V. Dragulyan Institute of Molecular Biology and Genetics, NAS of Ukraine Ukraine, 03143, Kyiv, str. Akad. Zabolotnogo 150
  • S. E. Rymar Institute of Molecular Biology and Genetics, NAS of Ukraine Ukraine, 03143, Kyiv, str. Akad. Zabolotnogo 150
  • V. A. Kordium Institute of Molecular Biology and Genetics, NAS of Ukraine Ukraine, 03143, Kyiv, str. Akad. Zabolotnogo 150
  • M. U. Levkiv Kievsky National University Taras Shevchenko Ukraine, 01601, Kyiv, Volodymyrska str, 64
  • R. V. Bubnov Institute of Microbiology and Virology. DK Zabolotny NAS of Ukraine Ukraine, 03143, Kyiv, str. Akad. Zabolotnogo 154; Tsentr ultrasound and sonography intervetsionnoy Clinical Hospital "Theophany" of the State Administration Ukraine, 036803, Kyiv, str. Akad. Zabolotnogo 21

Abstract

Aim. Getting a model of cirrhosis in male Wistar laboratory rats of different ages. Methods. Obtaining models of liver cirrhosis in rats of Wistar 3 and 8 months of age, and was carried out by intraperitoneal injection of 30% animal oil solution of CCl4 (0,01 ml/kg) 1 day a week. The dynamics of the disease studied biochemical (measurement of ALT, AST), cytology (blood count), histological and radiological (SPL) methods. Results. Based on the analysis of a model of cirrhosis in rats of different ages, from 3-month animals were observed formation of necrosis in the second week of liver fibrosis on the fourth week, finally cirrhosis formed on the second week of the experiment. The old 8-month rat or the 2nd or the 4th week CCl4 administration changes in the structure of the liver was observed. By the 6-week experiment histology showed the appearance of minor changes in the structure of the liver, similar to the beginning of the development of necrosis in the periphery of the body. Conclusions. Analysis of studying the characteristics of a model of cirrhosis of the liver in rats of different ages above mentioned methods can be considered animal model induced by 3 months of age, as close to human disease, and use it in the future for the development of more effective treatments for this disease.

Key words: hepatic necrosis, fibrosis, cirrhosis, rats Wistar, age factor.