The impact of androgenesis in vitro factors on wheat double haploid’s traits

Abstract

Aims. The influence of androgenesis in vitro factors on quantitative characters has been studied on winter bread wheat double haploids-lines differing in dwarfing genes. Methods. The parameters of agronomic traits of double haploids of three dwarf analogues of wheat vrieties and of cultivar Odesskaya 51 have been investigated comparing to the original forms with  the dwarfing gene alleles identified by PCR. Results. The significant differences between the parental forms and the double haploids towards height of plants, length of main spike and yield components have been established. Conclusions. The methods for developing of the androgenic double haploids was found to controversial change the expression of winter wheat characters depending on the specificity of lines, Rht-genotype, environment, and the number of generations ex vitro. For genotypes Rht8c Rht-B1e and Rht8c Rht-B1b, especially significant decline of the character parameters in doubled haploid lines with regard to length of stem and the main spike, number of spikelets in the ear were shown in both years, but most significantly – in the first generation. The lines with genotype Rht8c Rht-B1b were best discriminated in the space of studied traits. WTK variation had no relation to the androgenic effects and depended on the line peculiarities or the year conditions. The possibility of a misrepresentation of results of the selection of the breeding-valuable genotypes on account of the prolonged modification is discussed.

Key words: Triticum aestivum, androgenesis in vitro, double haploids, quantitative characters.