Effect of biotic elicitor and donor NO treatment in complex defence of wheat plants against hypoxia and wounding stress

  • I. V. Zhuk Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci., Ukraine, 03143, Kyiv, Aсad. Zabolotnogo str., 148 https://orcid.org/0000-0002-2496-2576
  • Ju. V. Shylina Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci., Ukraine, 03680, Kyiv, Aсad. Zabolotnogo str., 148
  • A. P. Dmytriev Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci., Ukraine, 03680, Kyiv, Aсad. Zabolotnogo str., 148
DOI: https://doi.org/10.7124/FEEO.v30.1464
Keywords: Triticum aestivum L., ferulic acid, hydrogen peroxide, hypoxia, wounding stress, NO

Abstract

Aim. The aim of the research was to investigate the induction of the Triticum aestivum L. non-specific tolerance by ferulic acid as a biotic elicitor and donor of nitric oxide as a signal molecule to hypoxia and wounding stress. Methods. Winter wheat plants cv. Kesariia Polis`ka were grown and had  treated by ferulic acid and sodium nitroprusside (SNP) solutions then wounding or flooding stress started. The hydrogen peroxide content was measured in leaves during the experimental period. Results. It is shown that an influence of the nitric oxide donor balanced the hydrogenous peroxide level in wounded leaves close to control. After two days of flooding stress plants treated by ferulic acid decreased hydrogen peroxide level on 15 % against threefold decreasing in non-treated stressed plants. Donor NO treatment also paid contribution to hydrogen peroxide equilibrium during the first hour under hypoxia conditions caused by flooding stress and decreased hydrogen peroxide content till the third day of stress. Conclusions. Ferulic acid and nitric oxide donor are perspective components for complex defense against hypoxia and wounding in wheat plants.

References

Zhuk І. V., Lisova G. M., Dmitriev A. P. Effects of oxalic acid and sodium nitroprusside on productivity and resistance of winter wheat to Septoria tritici leaf blotch and leaf rust infections.The Bulletin of Kharkiv national agrarian university. Series Biology. 2017. Vol. 2 (41). P. 68–76. [in Ukranian]

Zhuk І. V., Dmitriev A. P. Lisova G. M., Kucherova L.O. Participation of ferulic acid in elicitation of winter wheat plants resistance against Septoria tritici infection Factors in experimental evolution of organisms. 2017. Vol. 20. P. 190–193. doi: 10.7124/FEEO.v20.761. [in Ukranian]

Zhuk І. V., Dmitriev A. P. Lisova G. M., Kucherova L.O. The role of ferulic acid as a as biotic elicitor in elicitation of systemic resistance in Triticum aestivum against Alternaria spp. Studia biologica. 2017. Vol. 11 (3–4). P. 84–85. doi: 10.30970/sbi.1103. [in Ukranian]

Zhuk І. V., Dmitriev A. P. Lisova G. M., Kucherova L.O. The combination of NO donor and ferulic acid effect on the elicitation of Triticum aestivum tolerance against Septoria tritici Factors in Experimental Evolution of Organisms, 2018. Vol. 22. P. 240–245. doi: 10.7124/FEEO.v22.955. [in Ukranian]

Zhuk І. V., Dmitriev A. P. Lisova G. M., Kucherova L.O The influence of kojic acid and donor NO on Triticum aestivum L. under biotic stress. Factors in experimental evolution of organisms. 2019. Vol. 24. P. 219–224. doi: 10.7124/FEEO.v25.1166. [in Ukranian]

Zhuk І. V., Dmitriev A. P., Shylina Ju.V., Lisova G. M., Kucherova L.O. The estimation of organic acids effectiveness as biotic elicitors via changes of endogenous peroxide content Factors in experimental evolution of organisms. 2020. Vol. 26. P. 202–206. doi: 10.7124/FEEO.v26.1266. [in Ukranian]

Zhuk І. V., Shylina Ju.V., Dmitriev A. P. The activation of wheat resistance against powdery mildew by combination of biotic elicitor and NO donor. Factors in experimental evolution of organisms. 2021. Vol. 28. P. 78–82 doi: 10.7124/FEEO.v28.1379. [in Ukranian]

Chen L.-M., Kao Ch.-H. Effect of excess copper on rice leaves: evidence involvement of lipid peroxidation. Bot. Bull. Acad. Sin. 1999. Vol. 40. P. 283–287.

Hasanuzzaman M., Bhuyan M.H.M.B., Zulfiqar F., Raza A., Mohsin S.M., Mahmud J.A., Fujita M., Fotopoulos V. Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants (Basel). 2020. Vol. 29, 9 (8). P. 681. doi: 10.3390/antiox9080681.

Gupta K.J., Mur L.A.J., Wany A., Kumari A., Fernie A.R., Ratcliffe R.G. The role of nitrite and nitric oxide under low oxygen conditions in plants. New Phytol. 2020. Vol. 225 (3). P. 1143–1151. doi: 10.1111/nph.15969.

Loreti E., Striker G.G. Plant Responses to Hypoxia: Signaling and Adaptation. Plants (Basel). 2020. Vol. 9 (12). P. 1704. doi: 10.3390/plants9121704.

Loreti E., Perata P. The Many Facets of Hypoxia in Plants. Plants (Basel). 2020. Vol. 9 (6). P. 745. doi: 10.3390/plants9060745.

León, José et al. The hypoxia-reoxygenation stress in plants. Journal of experimental botany. 2021. Vol. 72 (16). P. 5841–5856. doi: 10.1093/jxb/eraa591.

Snoeck S., Guayazán-Palacios N., Steinbrenner A. D. Molecular tug-of-war: Plant immune recognition of herbivory. The Plant Cell. 2022. doi: 10.1093/plcell/koac009.

Pok B., Ngou M., Ding P., Jones J. D. G. Thirty years of resistance: Zig-zag through the plant immune system. The Plant Cell. 2022. doi: 10.1093/plcell/koac041.