Polymorphism of high-molecular-weight glutenin loci in Ukrainian wheat landraces Triticum aestivum L.

  • T. O. Sobko
  • G.M. Lisova
  • О.M. Blagodarova
DOI: https://doi.org/10.7124/FEEO.v29.1416

Abstract

Aim. The aim of the study was to investigate allelic variability of high-molecular-weight glutenin loci Glu-A1, Glu-B1, Glu-D1 in Ukrainian winter wheat landraces and obsolete cultivars Triticum aestivum L. Methods. Allelic diversity at the Glu-1 loci were analyzed in 54 collection accessions, including 41 landraces (Krymka, Banatka, Girka, Theyka and others), and 13 first breeding cultivars that were developed in the beginning of the last century by selection from local wheat. Method of SDS-PAG electrophoresis according to Laemmli was used for fractionation of HMW glutenin subunits. Results. A total 11 alleles at the Glu-1 loci were identified, including 3 alleles at the Glu-A1 (а, b, с) and
Glu-D1 (a, b, d) loci, and 5 – at the Glu- B1 (c, u, an, aj and subunit 9). Differences in frequencies of glutenin alleles were revealed. Conclusions. In the gene pool of Ukrainian winter bread wheat landraces the most widespread alleles were Glu-A1а (43.3 %), Glu-A1b (40.5 %), Glu-B1с (58 %), Glu-B1u (23 %), Glu-D1d (48.6 %), Glu-D1а (47.2 %). All these alleles (except of the Glu-D1a) are also predominant in the gene pool of modern commercial Ukrainian cultivars. A distinctive feature of Ukrainian landraces are the rare allelic variants of the Glu-B1 locus, which encode the subunits 1Ву9 and 1Ву8 (алель Glu-B1aj).

Keywords: Triticum aestivum L., winter wheat, landraces, high-molecular-weight glutenin, alleles.

References

Vavilov N. I. Nauchnye osnovy selektsii pshenitsy. M.-L.: 1935. 244 р. [in Russian]

Rabinovich S. V. Modern wheat varieties and their pedigrees: Kyiv: Urozhay, 1972. 328 p. [in Russian]

Lopes M. S., El-Basyoni I., Baenziger P. S., Singh S., Royo C., Ozbek K., Aktas H., Ozer E., Ozdemir F., Manickavelu A. Exploiting genetic diversity from landraces in wheat breeding for adaptation to climate change. J. Exp. Bot. 2015. Vol. 66 (12). P. 3477–3486. doi: 10.1093/jxb/erv 122.

Shewry P. R., Halford N. G., Belton P. S., Tatham A. S. The structure and properties of gluten: an elastic protein from wheat grain. Phil. Trans. R. Soc. Lond. B, 2002. 357. Р. 133–142. doi: 10.1098/rstb.2001.1024.

Payne P. I. Genetics of storage proteins and the effect of allelic variation on bread-making quality. Annual Review of Plant Physiology. 1987. Vol. 38. P. 141–153. http://dx.doi.org/10.1146/annurev.pp.38.060187.001041

Payne P. I., Lawrence G. J. Catalogue of alleles for the complex geneloci,Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res.Commun. 1983. Vol. 11 (1). P. 29–34.

Catalogue of gene symbols. Gene catalogue, 2013. Retrieved from: htpp://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jspMacGene.

Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. Vol. 227. P.680–685.

Lagudah, E. S., Flood R. G., Halloran G. M. Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghanistan. Euphytica. 1987. Vol. 36. P. 3–9.

Temizgul R., Akbulut M. Allele Frequency of Glutenin subunits and Glu-1 Quality Scores in Turkish Bread Wheat Landraces. Trakya Univ J Nat Sci. 2020. 21 (1). P. 1–11. doi: 10.23902/trkjnat.609487.

Gregová E., Hermunth J., Kraic J., Dotlačil L. Protein heterogenity in European wheat landraces and obsolete cultivars. Genet. Resour. Crop Evol. 1999. Vol. 46. P. 521–528. doi: 10.1023/A:1008751815445.

Gregová E., Hermunth J., Kraic J., Dotlačil L. Protein heterogeneity in European wheat landraces and obsolete cultivars: Additional information. Genet. Resour. Crop Evol. 2004. Vol. 51. P. 569–575. doi: 10.1023/B:GRES.0000024749.04012.ae.

Sobko T. O., Sozinov O. O. Characteristics of the Ukrainian winter common wheat varieties at the storage protein loci. Genetics and breeding in Ukrainian on the border of Millennium. Kyiv: Logos, 2001. Vol. 1. P. 151–159 [in Ukrainian].

Kozub N. A., Sozinov I. A., Karelov A. V., Blume Ya. B., Sozinov A. A. Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. Tsitologiya i genetika. 2017. Vol. 51 (2). P. 59–73. doi: 10.3103/S0095452717020050 [in Ukrainian].

Békés F., Cavanagh C.R., Martinov S., Bushuk W., Wrigley C.W. The Gluten Composition of Wheat Varieties and Genotypes Part III. Composition table for HMW-GS. 2006. Retrieved from: http://www.aaccnet.org/grainbin/pdfs/II_HMW_Subunits.pdf.