Evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols
Abstract
Aim. To evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols to optimize biotechnological approaches in pig reproduction based on the genetic material of the Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science of Ukraine. Methods. Cryopreserved ejaculated boars' sperm was thawed in three different ways. Biotechnological, cryobiological and morphological methods were used to assess the viability of the sperm. Results. We noted the individual feature of semen quality of the studied boars, which affects its suitability for cryopreservation and quality indicators after thawing. It was found that in liquefied boar sperm (sperm activity on average is 86.7%) after its centrifugation and three-hour equilibration at + 4 °C sperm activity decreased by an average of 25.0%. In order to improve the quality of cryopreserved sperm, it was thawed under different conditions. The highest activity of spermatozoa in thawed sperm of boar № 225 of Myrhorod pig was at the level of 25.0% at the thawing temperature + 70 °C, and the thermal resistance and heat resistance were 70.0% and 60.0%, respectively. Conclusions. During this study was noted that there are peculiarities of boar semen at breed and individual level, which influence its ability to tolerate cryopreservation.
Keywords: sperm, in vitro cultivation, cryobank, cryopreservation, boars.
References
Bwanga C.O. Cryopreservation of boar semen. I: a literature review. Acta Veterinaria Scandinavica. 1991. Vol. 32, No. 4. Р. 431–453.
Knox R.V. Artificial insemination in pigs today. Theriogenology. 2016. Vol. 85(1). P. 83–93. doi: 10.1016/j.theriogenology.2015.07.009
Rodriguez-Gil J.E., Estrada E. Bonet In S., Casas I., Holt W.V., Yeste M. Artificial insemination in boar reproduction. Boar reproduction. Berlin: Springer. 2013. Р. 589–608. doi: 10.1007/978-3-642-35049-8_12
Johnson L.A., Weitze K.F., Fiser P., Maxwell W.M.C. Storage of boar semen. Animal Reproduction Science. 2000. Vol. 62. P. 142–172. doi: 10.1016/S0378-4320(00)00157-3
Knox R.V. The fertility of frozen boar sperm when used for artificial insemination. Reproduction in Domestic Animals. 2015. Vol. 50 (2). Р. 90–97. doi: 10.1111/rda.12552
Bailey J.L., Lessard C., Jacques J., Brиque C., Dobrinski I., Zeng W., Galantino-Homer H.L. Cryopreservation of boar semen and its future importance to the industry. Theriogenology. 2008. Vol. 70. P. 1251–1259. doi: 10.1016/j.theriogenology.2008.06.014
Watson P.F., Behan J.R. Intrauterine insemination of sows with reduced sperm numbers: Results of a commercially based field trial. Theriogenology. 2002. Vol. 57. P. 1683–1693. doi: 10.1016/S0093-691X(02)00648-9
Korbets'ka O.O. Yakist' dekonservovanoi spermy knuriv zalezhno vid sposobiv ii zamorozhuvannia. Naukovo-tekhnichnyy biuleten' IT NAAN. 2013. No. 109, Pt. 1. P. 135−140. [in Ukrainian]
Kovtun S.I., Shcherbak O.V., Metlyts'ka O.I., Starodub L.F. Metodychni rekomendatsii z otsinky iakosti pryznachenoi do kriokonservatsii spermoproduktsii ta strukturnoi tsilisnosti spermatozoidiv knuriv. Chubyns'ke, 2018. 28 p. [in Ukrainian]
Lindgren Y., Lundeheim N., Boqvist S., Magnusson U. Reproductive performance in pigs reared under organic conditions compared with conventionally reared pigs. Acta Vet. Scand. 2013. Vol. 55. P. 33. doi: 10.1186/1751-0147-55-33.
Zel'din V.F., Shavkun Iu.N Osobennosti otsenki produktivnykh kachestv sviney v usloviiakh krupnomasshtabnogo proizvodstva svininy. Svinarstvo. 2010. Vip. 58. P. 24–31. [in Ukrainian]
Hladiy M.V., Polupan Yu.P., Kovtun S.I., Kuzebnij S.V., Vyshnevskiy L.V., Kopylov K.V., Shcherbak О.V. Scientific and organizational aspects of generation, genetics, reproduction biotechnology and protection of the genofonds in livestock breeding. Animal Breeding and Genetics. 2018. Vol. 56. P. 5–17. doi: 10.31073/abg.56.01.
