Screening cultivars of soybean, contrasting for rapidity maturity, of the degree for heat- and drought resistance, in vivo and in vitro
Abstract
Aim. Screening for complex heat and drought resistance in varieties of soybeans that differ for maturity in growing experiences and in vitro culture. Methods. To create the conditions of "artificial drought" experimental seedlings and calluses with the addition of 10% mannitol solution were cultured, heat resistance analysis out under the influence of temperature shock (60 ° C) of different duration (10-30 minutes) was carried. Drought and heat resistance by rapid analysis of seed germination and growth inhibition were determined, and in vitro by growth index (RI) and protein coagulation temperature (PCT). Results. During drought and hyperthermia tested varieties with different ripening differ in the degree of resistance to these stressors. Increased drought and heat resistance in conditions, both in vivo and in vitro middle-matured Yatran and middle-early Ustya, lowered – ultra-ripe Anushka were showed. The ripening Hajibey variety the lowest level of complex drought and heat resistance was characterized. Conclusions. It is assumed that the level of drought and heat resistance is determined by the genotypic characteristics of the varieties and may be related to the sign of the duration of the growing season – rapidity of maturity.
Keywords: Glycine max (L.) Merr., duration of growing season, drought resistance, heat resistance, callus culture.
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