Polymorphism of the moderate non-race-specific disease resistance gene Sr2/Lr27 and Lr34/Yr18/Pm38 in bread wheat cultivars of NSC «Institute of agriculture NAAS» breeding
Abstract
Aims. The object of our investigation is allelic state identification of Sr2/Lr27 and Lr34/Yr18/Pm38 gene in bread wheat cultivars of Polissia. Methods. Allelic state of the Lr34 gene was identified with the molecular-genetic marker caISBP1 use and marker for Sr2/Lr27 locus was csSr2. Results. In five cultivars (13,5 % of the total number) was detected the «Marquiz-allele» and rest had the «null allele» of Sr2 locus. The «resistant» allelic state of Lr34 gene (Lr34+) was identified in 11 cultivars (39 %). The «susceptible» allelic state of Lr34 was identified in 9 wheat cultivars (32 %) and 8 (29 %) cultivars showed polymorphism at the Lr34 locus. Conclusions. It is necessary to involve in wheat breeding process the Lr34 and Sr2 gene, because it is valuable source of disease resistance.
Key words: stem rust, Ug99, bread wheat, resistance, Sr2, Lr34.