Sequencing of cytochrome c oxidase subunit I (COI) gene and elongation factor 1-alpha (EF1α) gene in aphids of the Belarussian fauna

  • M. M. Varabyova
  • N. V. Voronova
DOI: https://doi.org/10.7124/FEEO.v25.1150

Abstract

Aim. Cytochrome c oxidase subunit I gene (COI) and elongation factor 1-alpha gene (EF1α) are frequently used for a correct species diagnostics of aphids forms that are correct diagnosis of the species, studying of species genetic structure, assessment of intraspecies and interspecies genetic polymorphism assessment and construction of phylogenetic systems. Methods. Aphids samples were collected in Belarus. Genetic sequences of COI and EF1α genes were sequenced using primes LCO and EF3. Results. Sequences of COI and EF1α genes of 18 aphid species of Belarussian fauna including 6 aphid species of COI gene (Aphis fabae mordvilkoi Börn. & Janisch,, Aphis pomi Deg., Aphis spiraecola Patch, Colopha compressa Koch, Panaphis juglandis (Gz.) и Uroleucon hypochoeridis (F.)) and 15 species of EF1α gene (Anoecia corni (Fabr.), Aphis euphorbiae Kalt., C. compressa, Drepanosiphum platanoidis (Schr.), Gyphina jacutensis Mordv., Hyalopterus pruni (Geoffr.), Longicaudus trirhodus (Walk.), Monaphis antennata (Kalt.), P. juglandis, Periphyllus aceris (L.), Schizolachmus pineti (F.), Sipha maydis Pass., Therioaphis tenera Aiz., Trichosiphonaphis corticis (Aiz.), U. hypochoeridis) were obtained. Conclusions. COI gene and EF1α gene sequences were decoded and deposited to GenBank.

Keywords: aphids, COI, EF1α, nucleotide sequence, GenBank, BOLD.

References

Cruickshank R.H. Molecular markers for the phylogenetics of mites and ticks. Systematic & Applied Acarology. 2002. Vol. 7, No. 1. P. 3–14. doi: 157.140.2.10/hosted_sites/acarology/saas/saa/pdf07/003-014.

Varabyova M.M. Morphological and genetic variability of aphid species (Sternorrhyncha: Aphidoidea) from different ecologi-cal and taxonomy groups: avtoref. dys. ... kand. biol. nauk. Мinsk, 2018. 28 p. [in Belarus]

Simon C., Frati F., Beckenbach A., Crespi B., Liu H., Flook P. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved PCR primers. Ann. Entomol. Soc. Am. 1994. Vol. 87, No. 6. P. 651–701. doi: hydrodictyon.eeb.uconn.edu/projects/cicada/resources/reprints/Simon_ea_1994.

Aravind К., Ravikanth G., Uma Shaanker R., Chandrashekara K., V. Kumar A.R., Ganeshaiah K.N. DNA barcoding : An ex-ercise in futility or utility? Current Science. 2007. Vol. 92, No. 9. P. 1213–1216. doi: jstor.org/stable/24097883.

GenBank Overview. USA. 2017. URL: https://www.ncbi.nlm.nih.gov/genbank/ (lаst accessed: 23.02.2019).

BOLD Systems v4. Ontario. 2017. URL: http:/www.barcodinglife.org/index.php/TaxBrowser_Home (lаst accessed: 23.02.2019).

Cho S., Mitchell A., Regier J.C., Mitter C., Poole R.W., Friedlander T.P., Zhao S. A highly conserved nuclear gene for low-level phylogenetics : Elongaton factor-1a recovers morphology based tree for heliothine moths. Mol. Biol. Evol. 1995. Vol. 12, No. 4. P. 650–656. doi: https://doi.org/10.1093/oxfordjournals.molbev.a040244.