Genetic structure of the Chernivtsi local stock of rainbow trout (Oncorhynchus mykiss) as determined by SSR-markers

  • O. Yu. Bielikova
  • O. Zaloilo
  • S. Тarasjuk
  • A. Mruk
  • V. Romanenko
DOI: https://doi.org/10.7124/FEEO.v25.1134

Abstract

Aim. The trend towards the development of high-tech trout farms is observed in Ukraine. The analysis of the genetic structure of rainbow trout by molecular genetic methods is necessary for breeding work. Therefore, the purpose of the study was to study polymorphism at the microsatellite loci of Oncorhynchus mykis of the chernivetsk local herd. Me-thods. The determination of the genetic polymorphism was carried out according to indicators: the effective number of alleles on the locus (ne), the observed (Но) and expected (Hе) heterozygosity, indexes of fixation (Fis) and polymorphism (PIC). Results. Specific features of the structure of the gene pool of the local herd O. mykiss by the SSR loci OMM 1032, 1077, 1088, STR 15, 60, 73 were revealed. The average value of the effective number of alleles per locus was 3.87. The high average values of the heterozygosity of the local herd and the index of fixation were fixed (mean values of H e and F is: 0.73 and -0.14, respectively). А high index of polymorphism was recorded for all used DNA markers and was 0.69. Тhe possibility to analyse the genetic structure of salmon using these SSR-markers was substantiated. Conclusions. An analysis of the genetic structure of trout (O. mykiss) was performed by using of 6 microsatellite. It was shown that the investigated local herd has a high level of heterozygosity. The conducted studies have shown the effectiveness of using selected loci for individual identification and population-genetic analysis.

Keywords: Oncorhynchus mykis, SSR-markers, heterozygotes, polymorphism.

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