Genetic potential of bacteria isolated from cadmium contaminated soils

  • O. S. Iungin
  • O. Yu. Bielikova
  • G. V. Gladka
  • O. B. Tashyrev
DOI: https://doi.org/10.7124/FEEO.v23.1040

Abstract

Aim. Determination of taxonomic position and resistance to Cd2+, Cu2+, Ni2+, Co2+, CrO42- of isolated bacteria from cadmium-contaminated soils. Methods. Bacterial cultures were isolated by ten-fold dilutions and further cultivation on agarized nutrient medium. Taxonomic position of isolated cultures was determined by molecular-genetic methods using GenBank and BLAST. Bacterial resistance to toxic metals was determined by strains cultivation in liquid medium in the presence of concentration gradient of Cd2+, Cu2+, Ni2+, Co2+, CrO42-. Results. Isolated cultures were highly resistance to cadmium. These bacterial cultures were identified as Brevundimonas vesicularis and Cupriavidus gilardii by phenotypic and molecular-genetic characteristics. Their resistance was in one order higher comparing to generally accepted. Conclusions. Isolated strains are promising for using in novel industrial biotechnologies for metal containing wastewater treatment.

Keywords: metalresistance, toxic metals, taxonomic position, Cupriavidus gilardii, Brevundimonas vesicularis.

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