Analysis of Glu-1 loci in samples of rare wheat species and their hybrides
Aim. Determination of the allelic composition of Glu-1 loci in samples of rare wheat species and their hybrids with spring bread wheat. Methods. PCR analysis. Results. In existing collection samples of the rare wheat species the alleles of the a/c loci Glu-A1 were found, while allele b was found only in T.dicoccum, var. volgense (Emmer Kokchetavskaya). An additional amplicon with a length of 450 bp was found in the Glu-A1 locus in the sample (T. dicoccum × Dasypyrum villosum), and in (Ae. ventricosa × T. dicoccum) another one a length of 700 bp in genome B, was found require further research. Among hexaploid wheat, it was revealed genotypes differing in the presence of alleles a and d of the locus Glu-D1. The hybridity of the received forms has been confirmed, as evidenced by the identification of both parent components in the hybrids or alleles of the Glu-D1 locus of bread wheat in hybrids with emmer. Conclusions. Analysis of the allele composition of Glu1 locuses in samples of rare wheat species and their hybrids with bread wheat allowed to select genotypes and hybrid combinations that may be promising for further breeding work.
Keywords: Triticum spelta L., T. dicoccum, hybrids, PCR analysis, Glu-1 locus.
Shelepov V.V., Gavrilyuk N.N., Vergunov V.A. Wheat: biology, morphology, breeding, seed production. Kiev: Logos, 2013. 498 p.
Campbell K.G. Spelt: agronomy, genetics, and breeding. Plant Breeding Rev. 1997. No. 15. P. 187–213.
Goncharov N.P., Kondratenko E.Ja. Wheat origin, domestication and evolution. Vavilov Journal of Genetics and Breeding. 2008. Vol. 12, No. 1-2. P. 159–179.
Chebotar S.V., Blagodarova E.M., Kurakina E.A. et al. Genetic polymorphism of loci determining bread making quality in ukrainian wheat varieties. Vavilov Journal of Genetics and Breeding. 2012. Vol. 16, No. 1. P. 87–98.
Payne P.I. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol Plant Mol Biol. 1987. No. 38. P. 141–153.
Simonov A.V., Pshenichnikova, T.A. Chromosomal localization of the speltoidy gene, introgressed into bread wheat from Aegilops speltoides Tausch., and its interaction with the Q gene of Triticum spelta L. Russ. J. Genet. 2012. Vol. 48, No. 11. P. 1120–1127.
Panchenko I.A., Ysova Z.V., Prutyla N.M. et al. Information value and inheritance of allelic variants of blocks of high molecular weight glutenins in the selection of winter wheat for grain quality. Plant breeding and seed production. 2007. Vol. 94. P. 115–128.
Rybalka A.I. The quality of wheat and its improvement. Kiev: Logos, 2011. 496 p.
Obreht D., Kobiljski B., Djan M., Vapa L. Identification of Glu-B1 alleles in bread wheat cultivars using PCR. Genetica. 2007. Vol. 39, No. 1. P. 23–28.
Liu S., Chao S., Anderson J. New DNA markers for high molecular weight glutenin subunits in wheat. Theor. Appl. Genet. 2008. Vol. 118. P. 177–183
Zhang Q., Dong Y.M., An X.L. et al. Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) J. Cereal Sci. 2008. Vol. 47. P. 252–261.