Cell selection with heavy metal ions for obtaining wheat and maize forms tolerant to osmotic stresses

  • L. E. Sergeeva
  • L. I. Bronnikova
DOI: https://doi.org/10.7124/FEEO.v22.968

Abstract

Aim. The obtaining tolerant to salt and water stresses wheat and maize variants via cell selection with barium and cadmium heavy metal cations were the idea. Barium cations disrupt the cell potassium transport. Cadmium cations affect the dehydrins. The maintenance of these parameters under stress conditions provides the plant tolerance. Methods. Modified media for initial selection were developed by the supplement of the lethal doses of heavy metal ions. Wheat and maize suspension cultures were plated on selective media. The relative fresh biomass growth was the marker of variant stress tolerance. Results. Ba-resistant and Cd‑resistant plant cell lines were obtained. Those variants were tested under salinity and water stress pressure at lethal for wild type cells doses. Cell lines demonstrated the combined tolerance to abiotic stresses. Conclusions. The cell selection with heavy metal ions – is a new approach for isolation cell variants with higher levels of osmotic stresses tolerance.

Keywords: cereals, osmotic stresses, cell selection with heavy metal ions, combined tolerance.

References

Maliga P. Isolation and characterization of mutants in plant cell culture. Ann. Rev. Plant Physiol. 1984. 35. P. 519–542. doi: 10.1146/annurev.pp.35.060184.002511

Dracup M. Why does in vitro cell selection not improve the salt tolerance of plants? Genetic aspects of plant mineral nutrition / Eds. Randall P.J. et al. Kluwer Academic Publishers, 1993. P. 137–142.

Nies D.H. Microbial heavy-metal resistance. Appl. Microbiol. Biotechnol. 1999. 51. P. 730–750. doi: 10.1007/s002530051457

Sergeeva L.E. Cell selection with heavy metal ions for obtaining plant genotypes with combined resistance to abiotic stresses. Kiev: Logos, 2013. 211 p.

Seregin I.V., Ivanov V.B. Physiological aspects of cadmium and lead toxic effects on the higher plants. Russ. J. Plant Phys. 2001. 48. P. 606–630.

Campbell S., Close T.J. Dehydrins: Genes, proteins and association with phenotypic traits. New Phytol. 1997. 137. P. 61–74. doi: 10.1046/j.1469-8137.1997.00831.x

Allagulova Ch.R., Gimalov F.R., Shakirova F.M., Vakhitov V.A. Plant dehydrins: structure and putative functions. Biochemistry (Moscow). 2004. 68. P. 1157–1165.

Hara M., Fujinaga M., Kuboi M. Metal binding by citrus dehydrin with histidine rich domains. J. Exp. Bot. 2005. 56. P. 2695–2703. doi: 10.1093/jxb/eri262

Fan L.-M., Wu W.-H., Yang Y.-Y. Identification and characterization the inward K+ channel in the plasma membrane of Brassica pollen protoplasts. Plant and Cell Physiol. 1999. 40, No. 8. P. 859–865. doi: 10.1093/oxfordjournals.pcp.a029615

Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962. 15. P. 473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x

Gamborg J.L., Miller R.A., Ojima K. Nutrient requirement of suspension cultures of soybean roots. Exp. Cell Res. 1968. 509. P. 151–158. doi: 10.1016/0014-4827(68)90403-5

Sidorov V.A. Plant biotechnology. Cell selection. Kiev: Naukova dumka, 1990. 280 p.

Sergeeva L.S. Changes of cell cultures under stress conditions. Kiev: Logos, 2001. 100 p.

Meredith C.P. Selection an d characterization of aluminum-resistant variants from tomato cell cultures. Plant Sci. Lett. 1978. 12. P. 25–34.

Dziubinska H., Filek M., Krol E., Trebacz K. Cadmium and selenium modulate slow vacuolar channels in rape (Brassica napus) vacuoles. J. Plant. Physiol. 2010. Vol. 167. P. 1566–1570. doi: doi: 10.1016/j.jplph.2010.06.016

Bondgaard M., Bjerregaard P. Association between cadmium and calcium uptake and distribution during the moult cycle of female shore crabs Carcinus maenas: an in vivo study. Aquat. Toxicol. 2005. 72. P. 17–28. doi: 10.1016/j.aquatox.2004.11.017

Melekhov E.I., Anev V.N. Reversible efflux of К+ from cell as a defense reaction to unfavourable conditions. J. Gen. Biology (Moscow). 1991. vol. 52. P. 14–26

Hasegawa P.M., Bressan R.A., Zhu J.-K., Bohnert H.J. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 2000. 51. P. 463–499. doi: 10.1146/annurev.arplant.51.1.463