The frequency of T-DNA integration during the genetic transformation of tobacco (Nicotiana tabacum L.)

  • A. G. Komisarenko
  • S. I. Mykhalska
  • O. O. Khrystan
DOI: https://doi.org/10.7124/FEEO.v22.959

Abstract

Aim. Investigation of the is integration frequency of the genes and their components (elements of genetic constructions) in transgenic tobacco-regenerating plants. Methods. Agrobacterium-mediated transformation of tobacco (Nicotiana tabacum L.) in vitro. Results. There were obtained tobacco plants with integrated target and selective genes. It was found that a complete copy of T-DNA or its certain elements were embedded. Conclusions. The possibility of the integration of complete and incomplete DNA copies during in vitro Agrobacterium-mediated transformation of the Nicotiana tabacum were established. This event were observed both during manipulations with pBi2E plasmid with the double-stranded RNA PHD gene suppressor and pBi-OAT plasmid with OAT gene. The transformation frequency with the complete integration of the genetic construction was 91 %. The integration frequencies for various plasmids were: LBA4404 pBi2E – 78 %; AGLO pBi2E – 100 %; AGLO pBi-OAT – 94 %.

Keywords: Nicotiana tabacum L., Agrobacterium-mediated transformation in vitro, transgenic plants, T-DNA.

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