Intravarietal intron-length polymorphism of β-tubulin genes in belorussian landraces of Linum usitatissimum L.

  • A. M. Rabokon
  • Ya. B. Pirko
  • L. O. Kalafat
  • Ye. V. Guzenko
  • M. V. Bogdanova
  • V. I. Sakovich
  • V. A. Lemesh
  • Ya. B. Blume
DOI: https://doi.org/10.7124/FEEO.v22.945

Abstract

Aim. The aim of the work was to evaluate the possibilities of using the β-tubulin intron length (TBP, Tubulin Based Polymorphism) for genetic differentiation of ancient flax varieties (landraces), plants that was historically formed in Belarus. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments (β-tubulin introns) were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The size of the amplified fragments varied from 400 bp to 1900 bp. It was found that 25 of 30 studied varieties (landraces) were genetically heterogeneous. The total number of allele phenotypes was 7, and the value of PIC (Polymorphism Information Content) varied from 0.0 to 0.72. Conclusions. The data obtained make it possible to recommend landraces as a source of genes for increasing the genetic diversity of the existing flax gene pool, and the TBP method can be applied both in molecular-phylogenetic analysis and in molecular selection of flax.

Keywords: Linum usitatissimum, landrace, molecular markers, DNA fingerprinting, β-tubulin genes, intron polymorphism.

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