Genotyping of the site of the mtDNA of representatives of the Cetoniinae subfamily

  • N. A. Balashenko
  • A. A. Semenyak
  • A. S. Kornilkova
  • I. A. Sen'kevich
  • O. V. Prishchepchik
  • S. E. Dromashko
DOI: https://doi.org/10.7124/FEEO.v22.932

Abstract

Aim. Description of the results of molecular genetic research of insects-representatives of the subfamily Cetoniinae (flower chafer). Methods. For the analysis, methods of low-traumatic sampling of material for DNA isolation, polymerase chain reaction method, sequencing of the region of mtDNA COI gene were used. Results. A technique for sampling biological material for isolating DNA from the hind legs of individuals was proposed, which allowed not to remove from the habitat the individuals of the most valuable species, collected on the territory of the Republic of Belarus. A collection of DNA samples of representatives of the subfamily Cetoniinae was prepared. The haplotypes Oxythyrea funesta (Poda, 1761) and Protaetia aeruginosa (Linnaeus, 1767) of representatives of the populations inhabiting the territory of Belarus have been identified. Conclusions. There were significant differences in haplotypes among representatives of the Belarusian and European parts of their areal.

Keywords: molecular markers, mtDNA, DNA-barcoding, insects, subfamily Cetoniinae.

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