Modeling of Parkinson's disease on D. melanogaster: oxidative stress and the role of isogenization of transgenic lines

  • Kh. A. Dronska
  • Kh. M. Yavdyk
  • O. H. Stasyk
  • N. P. Matiytsiv
DOI: https://doi.org/10.7124/FEEO.v22.922

Abstract

Aim. Oxidative stress (OS) is considered one of the main factors that leads to the degeneration of dopamine neurons in Parkinson's disease (PD). The purpose of the work was to test sensitivity to the conditions of the OS of D. melanogaster individuals with expression of human alpha-synuclein in neurons UAS-SNCA/elavGal4; and establish the role of the isogenization of the lines derived from stock collections in the study of this phenotype. Methods. For the isogenyzation of the line, we conducted five generations of sequential crossings of individuals with insertion of human alpha synuclein gene into the w1118 line. A 4-day test using H2O2 as prooxidant was used to test the sensitivity to OS conditions. Results. Individuals with expression of alpha-synuclein gene in neurons were characterized by statistically significant sensitivity to OS conditions, compared with controls. Also, there was a significant difference in the degree of sensitivity to the OS in the second day of the experiment in individuals before and after the isogenization of the effector line. Conclusions. Hypersensitivity to the OS is detected as a specific phenotype under conditions of expression of human alpha-synuclein in Drosophila neurons. The importance of the isogenization of transgenic lines for the characterization of the stress susceptibility phenotype is established.
Keywords: Drosophila melanogaster, alpha-synuclein, oxidative stress, isogenization.

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