Verification allelic polymorphism's of genes MTHFR and MTR in patients with psoriasis

  • A. M. Fedota
  • L. V. Roshcheniuk
  • T. V. Tyzhnenko
  • A. V. Admakina
  • I. V. Horaichuk
  • V. M. Vorontsov
DOI: https://doi.org/10.7124/FEEO.v21.865

Abstract

Aim. Analysis of single nucleotide polymorphisms C677T, A1298C and A2756G of MTHFR and MTR one-carbon metabolism genes in patients with various forms of psoriasis in Ukrainian population. Methods. A molecular genetic analysis of 77 patients with vulgaris and arthropathic psoriasis by PCR-RFLP was carried out. Results. In patients with vulgaris and arthropathic psoriasis analysis of the distribution of frequencies of genotypes showed a statistically significant difference between them for C677T polymorphic variants. In patients with psoriasis analysis of genotype distribution of series in the two genes as a whole, showed a statistically significant difference between the theoretically expected and actual frequencies for single nucleotide polymorphisms A1298T and A2756G of MTHFR and MTR genes. Conclusions. Among patients with arthropatic psoriasis, which is the most severe form of psoriasis, the homozygotes for the wild-type allele G of A2756G of MTR gene are more rarely, homozygotes of the TT of C667T of MTHFR gene are found more common than among psoriasis vulgaris patients, which may indicate the contribution of other genes to the development of arthropatic psoriasis.

Keywords: psoriasis, arthropatic psoriasis, folate cycle, MTHFR gene, MTR gene.

References

Brynina A.V, Khvorik D.F. Rol' triggernykh faktorov v patogeneze psoriaza, assotsiirovannogo s kardiovaskuliarnoy patologiey. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2015. No. 4. P. 10-14. [in Russian]

López-Cortés A., Jaramillo-Koupermann G., J. Muñoz M., Cabrera A., Echeverría C., Paz-y-Miño C. Genetic рolymorphisms in MTHFR (C677T, A1298C), MTR (A2756G) and MTRR (A66G) genes associated with pathological characteristics of prostate cancer in the ecuadorian population. The American Journal of the Med Sciences. 2013. V. 346(6). P. 447-454. doi: 10.1097/MAJ.0b013e3182882578

De Cássia R., Barbosa C., Menezes Da Costa D., Francelino Cordeiro D.E., Freitas Vieira A.P., Barem Rabenhorst S.H. Interaction of MTHFR C677T and A1298C, and MTR A2756G gene polymorphisms in breast cancer risk in a population in northeast Brazil. Anticancer research. 2012. No. 32. Р. 4805-4812.

Qin X., Li J., Cui Y., Liu Z., Zhao Z., Ge J., Guan D., Hu J., Wang Y., Zhang F., Xu Х., Wang X., Xu X., Huo Y. MTHFR C677T and MTR A2756G polymorphisms and the homocysteine lowering efficacy of different doses of folic acid in hypertensive Chinese adults. Nutrition Journa. 2012. No. l. Р. 2-11. doi: 10.1186/1475-2891-11-2

Weinera A.S., Boyarskikha U.A., Voronina E.N., Mishukova O.V., Filipenko M.L. Methy-lenetetrahydrofolate reductase C677T and methionine synthase A2756G polymorphisms influence on leukocyte genomic DNA methylation level. Gene. 2014. V. 533(1). P. 168-172. doi: 10.1016/j.gene.2013.09.098

Zhao J.Y., Qiao B., Duan W.Y., Gong X.H., Peng Q.Q., Jiang S.S., Lu C.Q., Chen Y.J., Shen H.B., Huang G.Y., Jin L., Wang H.Y. Genetic variants reducing MTR gene expression increase the risk of congenital heart disease in Han Chinese populations. Eur Heart J. 2014. V. 35(11). Р. 42-733. doi: 10.1093/eurheartj/eht221

Kurzawski M., Wajda A., Malinowski D., Kazienko A., Kurzawa R., Drozdzik M. Association study of folate-related enzymes (MTHFR, MTR, MTRR) genetic variants with non-obstructive male infertility in a Polish population. Genet. Mol. Biol. 2015. V. 38(1). Р. 42-47. doi: 10.1590/S1415-475738120140179

Wu D., Shi D., Yang L., Zhu X. Association between methylenetetrahydrofolate reductase C677T polymorphism and psoriasis: A meta-analysis. J Dermatol. 2016. No. 43. Р. 162-169. doi: 10.1111/1346-8138.13039

Vasku V., Bienertova-Vasku J., Necas M., Vasku A. MTHFR (methylenetetrahydrofolate reductase) C677T polymorphism and psoriasis. ClinExp Med. 2009. № 9. Р. 31-327. doi: 10.1007/s10238-009-0054-0

Asefi M., Vaisi-Raygani A., Khodarahmi R., Nemati H., Rahimi Z., Vaisi-Raygani H., Tavilani H., Pourmotabbed T. Methylentetrahydrofolatereductase (rs1801133) polymorphism and psoriasis: contribution to oxidative stress, lipid peroxidation and correlation with vascular adhesion protein 1, preliminary repor. J. Eur. Acad. DermatolVener. 2014. No. 28. Р. 8-1192. doi: 10.1111/jdv.12262

Karabacak E., Aydin E., Ozcan O., Dogan B., Gultepe M., Cosar A., Muftuoglu T. Methylenetetrahydrofolate reductase (MTHFR) 677C>T gene polymorphism as a possible factor for reducing clinical severity of psoriasis. Int. J. Clin. Exp. Med. 2014. V. 7 (3). Р. 697-702.

Kilic S., Ozdemir O., Silan F., Isik S., Yildiz O., Karaagacli D., Silan C., Ogretmen Z. Possible association between germline methylenetetrahydrofolate reductase gene polymer-phisms and psoriasis risk in a Turkish population. ClinExpDerma. 2015. No. 42. Р. 8-13. doi: 10.1111/ced.12909

Qiao J.H., Jiao D.C., Lu Z.D., Cui S.D., Liu Z.Z. Association of methylenetetrahydrofolate reductase and methionine synthase polymorphisms with breast cancer risk and interaction with folate, vitamin B6, and vitamin B12 intakes. Tumour. Biol. 2014. No. 35. Р. 11895-11901. doi: 10.1007/s13277-014-2456-1

Atramentova L.O., Utievs'ka O.M. Statystychnimetody v biolohii. Kh.: KhNU, 2007. 288 p. [in Ukrainian]

Weiwei Z., Liping C., Dequan L. Association between dietary intake of folate, Vitamin B6, B12 and MTHFR, MTR and breast cancer risk. Pak. J. Med. Sci. 2014. No. 30 (1). Р. 106-110.

Zhang J., Zhou Y.W., Shi H.P., Wang Y.Z., Li G.L., Yu H.T., Xie X.Y. 5,10-Methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTRR), and methionine synthase reductase (MTR) gene polymorphisms and adult meningioma risk. J Neurooncol. 2013. No. 115 (2). Р. 9-233. doi: 10.1007/s11060-013-1218-z

Li W.-X., Dai S.-X., Zheng J.-J., Liu J.-Q., Huang J.-F. Homocysteine metabolism gene polymorphisms (MTHFR C677T, MTHFR A1298C, MTR A2756G and MTRR A66G) jointly elevate the risk of folate deficiency. Nutrients. 2015. V. 7 (8). Р. 6670-6687. doi: 10.3390/nu7085303

Kofink D., Boks M.P., Timmers H., Kas M.J. Epigenetic dynamics in psychiatric disorders: environmental programming of neurodevelopmental processes. NeurosciBiobehav Rev. 2013. No. 37. Р. 45-831. doi: 10.1016/j.neubiorev.2013.03.020

Baiqiu W., Songbin F., Guiyin Z., Pu L. Study of the relationship between psoriasis and the polymorphic site C677T of methylenetetrahydrofolate reductase. Chin Med Sci J. 2000. № 15. Р. 20-119.

Zhao M., Wang L.T., Liang G.P., Zhang P., Deng X.J., Tang Q., Zhai H.Y., Chang C.C., Su Y.W., Lu Q.J. Up-regulation of microRNA-210 induces immune dysfunction via targeting FOXP3 in CD4T cells of psoriasis vulgaris. ClinImmunol. 2013. No. 150. Р. 22-30. doi: 10.1016/j.clim.2013.10.009