Cardiac deletion of α-Е-catenin leads to redused expression of the main component of desmosomes – γ-catenin

  • V. V. Balatskyi
  • L. L. Matsevych
  • O. O. Piven
DOI: https://doi.org/10.7124/FEEO.v21.854

Abstract

Aim. In our present work, we have addressed to the γ-catenin, known main component of desmosomes, expression in hearts with heterozygous and homozygous knockout of α-E-catenin gene. Methods. Alpha-E-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. We analyze expression of γ-catenin with real time qPCR and Western blot. Results. Cardiac α-E-catenin deletion leads to downregulation of γ-catenin mRNA and protein levels only in homozygous mice, while we not observed any perturbation of γ-catenin expression in heterozygous mice. Conclusions. We have shown that homozygous knockout of α-E-catenin gene in embryonic heart occur reduction of the main component of desmosomes – γ-catenin mRNA and protein level of expression, which can lead to disruption of the desmosomes structure in adult myocardium.

Keywords: α-E-catenin, heart failure, γ-catenin.

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