Microsatellite locus Ame17 polymorphism for round goby (Neogobius melanostomus) from Northwest Black Sea region
Abstract
Aim. To identify the Ame17 polymorphic variants for round goby Neogobius melanostomus from Northwest Black Sea region. Methods. We determined polymorphism for 102 individuals of round goby. Genotyping was performed by PCR-analysis of Ame17 as described by Brown et al., 2008. The PCR-amplified DNA products were subjected to electrophoresis in 8 % polyacrylamide gel. Results. There have been identified 23 alleles. The range of repeat sizes was from 140 to 300 bp. The average heterozygosity was 0.76. Conclusions. There have been analyzed polymorphisms of Ame17 microsatellite locus for round goby from Northwest Black Sea region. The highest genetic diversity at Ame17 locus was observed for fish from the Gulf of Odessa. In terms of heterozygosity subpopulation of round goby from Bay Dzharylgach was significantly different from other localities.
Keywords: round goby, polymorphism, microsatellite loci, Ame17.
References
Tkachenko M.Yu. Morfolohichna minlyvist' bychka-kruhliaka Neogobius melanostomus (Pallas, 1814) mors'kykh ta prisnovodnykh vodoym. Biolohichni systemy. 2012. V. 4(4). P. 525-529. [in Ukrainian]
Brown J.E., Stepien C.A. Invasion genetics of the Eurasian round goby in North America: tracing sources and spread patterns. Mol. Ecol. 2009. V. 18. P. 64-79. doi: 10.1111/j.1365-294X.2008.04014.x
Kovacic M., Engin S. Two new species of Neogobius (Gobiidae) from northeastern Turkey. Cybium. 2008. V. 32. P. 73-80.
Feldheim K.A., Willink P., Brown J.E., Murphy D.J., Neilson M.E., Stepien C.A. Microsatellite loci for Ponto-Caspian gobies: markers for assessing exotic invasions. Mol. Ecol. Res. 2009. V. 9. P. 639-644. doi: 10.1111/j.1755-0998.2008.02495.x
Skora K.E., Stolarski J. New fish species in the Gulf of Gdansk, Neogobius sp. [cf. Neogobius melanostomus (Pallas 1811)]. Bulletin of the Sea Fisheries Institute. 1993. V. 1. P. 83-84.
Jude D.J., Reider R.H., Smith G.R. Establishment of Gobiidae in the Great Lakes basin. Canadian Journal of Fisheries and Aquatic Sciences. 1992. V. 49. P. 416-421. doi: 10.1139/f92-047
Hensler S.R., Jude D.J. Diel vertical migration of round goby larvae in the Great Lakes. Journal of Great Lakes Research. 2007. V. 33. P. 295-302. doi: 10.3394/0380-1330(2007)33[295:DVMORG]2.0.CO;2
Brown J.E., Stepien C.A. Ancient divisions, recent expansions: phylogeography and population genetics of the round goby Apollonia melanostoma. Molecular Ecology. 2008. V. 17. P. 2598-2615. doi: 10.1111/j.1365-294X.2008.03777.x
Poulin J., Weller S.G., Sakai A.K. Genetic diversity does not affect the invasiveness of fountain grass (Pennisetum setaceum) in Arizona, California and Hawaii. Diversity and Distributions. 2005. V. 11. P. 241-247. doi: 10.1111/j.1366-9516.2005.00136.x
Frankham R. Resolving the genetic paradox in invasive species. Heredity. 2005. V. 94. P. 385. doi: 10.1038/sj.hdy.6800634
Smith L.D. Ch. 10. The role of phenotypic plasticity in marine biological Invasions / Eds Rilov G., Crooks J.A.. Biological invasions in marine ecosystems. Ecological Studies. Berlin; Heidelberg: Springer, Verlag. 2009. P. 177-202. doi: 10.1007/978-3-540-79236-9_10
Nohara K., Kokita T., Tominaga O., Seikai T. Isolation and characterization of 11 polymorphic microsatellite loci in the whitegirdled goby (Pterogobius zonoleucus) and cross-species amplification in the serpentine goby (P. elapoides). Mol. Ecol. Res. 2009. V. 9. P. 610-612. doi: 10.1111/j.1471-8286.2009.00228_9_2.x
Atramentova L.A., Utevskaia O.M. Statisticheskie metody v biologii. Gorlovka: Lіkhtar, 2008. 248 p. [in Russian]
Slyn'ko Iu.V., Stolbunova V.V., Chebotar' S.V., Zamorov V.V., Gurovskiy A.N. Raznoobrazie gaplotipov po lokusu cyt b mtDNK bychka-krugliaka (Neogobius melanostomus (Pallas)) v severo-zapadnoy chasti Chernomorskogo basseyna. Genetika, 2014. V. 50(3). P. 314-320. [in Russian] doi: 10.1134/S1022795414030090
