Effects of non – allelic interactions of maize endosperm structure genes on the oil fatty acid composition

  • D. S. Tymchuk
  • V. V. Muzhylko
  • S. M. Tymchuk
DOI: https://doi.org/10.7124/FEEO.v21.836

Abstract

Aim. Determination of effects of the non-allelic interactions between the different genes of maize endosperm structure and identification the carriers of combinations of mutant genes with the best oil fatty acid compositions. Methods. The representative complex of maize inbreds – carriers of mutant genes о2, sh1, sh2, su1, su2, ae and wx and their paired combination was used as the material for research. Oil fatty acid composition was analysed by the modified gas – chromatographic Peysker method. Statistical analysis of the results was performed by the method of variance analysis. Results. The carriers of endospermic mutation su1 were notable as having the most high content of oleate in oil and the carriers of mutation sh2 – as having the most high palmitate content. The non–allelic interactions of endosperm structure genes for the oil fatty acid composition were realized by three types. The first of them took place immediate level of components of the oil fatty acid composition between the mutants who were partners of the combination. The second type was characterized by the presence of epistatic deviation of some components of the fatty acid composition in the direction of one the monogenic partners of combination. In the third type it has been observed the synergistic increase or reduction of the components of the fatty acid composition compared with both partners of combination. Conclusions. Use of non–allelic interactions between the mutant genes of maize endosperm structure can be observed as an effective method of the extension of useful genetic diversity while improving the oil quality. The most significant beneficial effects of non – allelic interactions of the synergistic type were provided by the combinations involving the mutant genes su1 and sh2.
Keywords: maize, endospermic mutants, non-allelic interactions, oil fatty acid composition.

References

Boyer C.D., Hannah L.C. Kernel mutants of corn. Specialty Corns; A.R. Hallauer Ed. Boca Raton – London – New-York – Washington, D.C.: CRC Press, 2001. P. 10–40.

Pajic Z. Breeding of maize types with specific traits at the Maize Research Institute, Zemun polje. Genetika. 2007. V. 39. P.169-180. doi: 10.2298/GENSR0702169P

Balconi C., Hartings H., Lauria M., Pirona R., Rossi V., Motto M. Gene discovery to improve maize grain quality traits. Maydica. 2007. V. 52. P. 357-373.

Flora L.F., Wiley R.C. Effect of various endosperm mutants on oil content and fatty acid composition of whole kernel corn (Zea mays L.). J. Amer. Soc. Hortic. Sci. 1972. V. 97. P. 604-607.

Moreau R.A. Corn oil. Bailey's industrial oil anf fat products; F. Shahidi Ed. 6th ed. Hoboken,New Jersey: Wiley - Intersci. Publ, 2005. V. 2. P. 149-172. doi: 10.1002/047167849X.bio007

Lambert R.J. High-oil corn hybrids. Specialty Corns; A.R. Hallauer Ed. Boca Raton - London - New York - Washington, D.C.: CRC Press, 2001. P. 137-161. doi: 10.1201/9781420038569.ch5

White P.J., Pollak L.M., Duvick S. Improving the fatty acid composition of corn oil by using germplasm introgression. Lipid Technol. 2007. V. 19. P. 35-38. doi: 10.1002/lite.200600009

Lee E.A. Maize for oil. Oil crops; J. Vollmann, I. Raican Eds. Dordrecht - Heidelberg - London - New-York: Springer Sci., 2009. Р. 493-506. doi: 10.1007/978-0-387-77594-4_17

White P.J., Weber E.J. Lipids of the kernel. Corn: Chemistry and Technology, 2nd Ed.; P.J. White, L.A. Johnson Eds. 2nd ed. St. Paul, MN: Amer. Assoc. Cereal Chem. 2003. P. 355-395.

Scrimgeour C. Chemistry of fatty acids. Bailey's industrial oil anf fat products; F. Shahidi Ed. 6th ed. Hoboken, New Jersey: Wiley - Intersci. Publ, 2005. V. 1. P. 1-43. doi: 10.1002/047167849X.bio005

Moreau R.A. Corn oil. Vegetable oils in food technology: composition, properties and uses; F.D. Gunstone Ed. 2nd ed. Chichester: Wiley & Blackwell, 2011. P. 273-289. doi: 10.1002/9781444339925.ch10

Vanhercke T., Wood C.C., Stymne S., Singh S.P., Green A.G. Metabolic engineering of plant oils and waxes for use as industrial feedstocks. Plant Biotechnol. J. 2013. V. 11 - P. 197-210. doi: 10.1111/pbi.12023

Vasal S.K. The quality protein maize story. Food and Nutr. Bull. 2000. V. 21. P. 445-450. doi: 10.1177/156482650002100420

Dang N.C., Munsch M., Aulinger I., Renlai W., Le-Huy H., Jampaton S., Stamp P. Composition of starch and protein in the endosperm of newly generated double recessive waxy and opaque-2 maize (Zea mays L.) genotypes. J. Agr. Sci. Technol. 2011. V. 1. P. 631-637.

Tymchuk S.M., Nikolenko I.A. Sposib oderzhannia endospermovykh rekombinantiv kukurudzy. Patent Ukrainy na korysnu model' No 20433 vid 15.01.2007. Biul. No 1. [in Ukrainian]

Dospekhov B.A. Metodika polevogo opyta. Moskva: Agropromizdat, 1985. 351 p. [in Russian]

Neuffer M.G., Coe E.H., Wessler S.R. Mutants of maize. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1997. 468 p.

Prokhorova M.I. Metody biokhimicheskikh issledovaniy. Leningrad: Khimiia, 1982. 272 p. [in Russian]

Lakin G.F. Biometriia. Moskva: Vysshaia shkola, 1973. 343 p. [in Russian]

Alrefai R., Berke T.G., Rocheford T.R. Quantitative trait locus analysis of fatty acid concentrations in maize. Genome. 1995. V. 38. P. 894-901. doi: 10.1139/g95-118

Motto M., Balconi C., Hartings H., Rossi V. Gene discovery for improvement of kernel quality-related traits in maize. Genetika. 2010. V. 42. P. 23-56. doi: 10.2298/GENSR1001023M

Belo A., Zheng P., Luck S., Shen B., Meyer D.J., Li B., Tingey S., Rafalski A. Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize. Mol. Genet. Genom. 2008. V. 279. P. 1-10. doi: 10.1007/s00438-007-0289-y