Analysis of Glu-1 loci in hybrid Triticum spelta L. × Triticum aestivum L.

  • S. M. Sichkar
  • L. H. Velykozhon
  • O. V. Dubrovna
DOI: https://doi.org/10.7124/FEEO.v21.833

Abstract

Aim. Determination of allelic status of genes in samples of winter spelt and bread wheat varieties and their interspecific hybrids, as well as a comparative analysis of specific combinations of loci and characteristics of grain quality. Methods. We used the method of multiplexed PCR. Results. DNA analysis of allelic status of genes of loci Glu-A1 and Glu-D1 in samples of winter spelt and their interspecific hybrids with bread wheat samples allowed to isolate spelt and hybrid combinations that could be promising for further breeding. It was shown that the allele d of locus Glu-D1 improves the baking properties of hybrids. Conclusions. Genetic improvement spelt by interspecific hybridization with varieties of bread wheat that contain only d allele of locus Glu-D1 can give rise to lines with high protein and good baking qualities.
Keywords: Triticum spelta L., T. aestivum L., PCR, loci Glu-1, PCR-analysis.

References

Jorgensen J.R., Olsen C.C. Yield and quality assessment of spelt (Triticum spelta L.) compared with winter wheat (Triticum aestivum L.) in Denmark. Spelt and Quina. Working Group Meeting (24–25 Oct. 1997). Wageningen, the Netherlands, 1997. P. 33–38.

Eltun R., Aasven M. The possibilities for spelt cultivation in Norway. Spelt and Quina. Working Group Meeting (24–25 Oct. 1997). Wageningen, the Netherlands, 1997. P. 7–13.

Dahlstedt L. Spelt Wheat (Triticum aestivum ssp. spelta (L.)) An alternative crop for ecological farming systems. Spelt and Quina. Working Group Meeting (24–25 Oct. 1997). Wageningen, the Netherlands, 1997. P. 3–6.

Galova Z., Knoblochova Н. Biochemical characteristics of five spelt wheat cultivars (Triticum spelta L.). Acta fytotechnica et zootechnica. 2001. V. 4. P. 85–87.

Campbell K.G. Spelt: agronomy, genetics, and breeding. Plant Breeding Rev. 1997. No. 15. P. 187-213. doi: 10.1002/9780470650097.ch6

Shelepov V.V., Gavriliuk N.N., Vergunov V.A. Pshenitsa: biologiia, morfologiia, selektsiia, semenovodstvo. Kyiv: Logos, 2013. 498 p. [in Russian]

Chebotar' S.V., Blagodarova E.M., Kurakina E.A., Semeniuk I.V., Polishchuk A.M., Kozub N.A., Sozinov I.A., Khokhlov A.N., Rybalka A.I., Sivolap Iu.M. Geneticheskiy polimorfizm lokusov, opredeliaiushchikh khlebopekarnoe kachestvo ukrainskikh sortov pshenitsy. Vavilovskiy zhurnal genetiki i selektsii. 2012. V. 16(1). P. 87-98. [in Russian]

Payne P.I. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol Plant Mol Biol. 1987. V. 38. P. 141-153. doi: 10.1146/annurev.pp.38.060187.001041

Niniieva A.K., Kozub N.O., Sozinov I.O., Rybalka O.I., Leonov O.Yu., Tverdokhlib O.V., Bohuslavs'kyy R.L. Kharakterystyka zrazkiv Triticum spelta L. za pokaznykamy iakosti zerna ta elektroforetychnymy spektramy zapasnykh bilkiv. Visnyk ukrains'koho tovarystva henetykiv i selektsioneriv. 2013. V. 11(1). P. 96-105. [in Ukrainian]

Romanova Iu.A., Gubareva N.K., Konarev A.V., Mitrofanova O.P., Liapunova O.A., Anfilova N.A., Strel'chenko P.P. Issledovanie kollektsii vida pshenitsy Triticum spelta L. po polimorfizmu gliadinov. Genetika. 2001. V. 37(9). P. 1258-1265. [inRussian] doi: 10.1023/A:1011965615619

Kozub N.A., Boguslavskiy R.L., Sozinov I.A., Tverdokhleb E.V., Ksinias I.N., Blium Ia.B., Sozinov A.A. Alleli po lokusam zapasnykh belkov u obraztsov Triticum spelta L. i ikh vstrechaemost' u rodstvennykh pshenits. Tsitologiia i genetika. 2014. V. 48(1). P. 41-51. [in Russian] doi: 10.3103/S0095452714010046

An X., Qiaoyun L., Yueming Y., Yinghua X., Hsam S.L., Zeller F.J. Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci. Euphytica. 2005. V. 146. P. 193-201. doi: 10.1007/s10681-005-9002-6

Obreht D., Kobiljski B., Djan M., Vapa L. Identification of Glu-В1 alleles in bread wheat cultivars using PCR. Genetica. 2007. V. 39(1). P. 23-28. doi: 10.2298/GENSR0701023O

Gale K.R. Diagnostic DNA markers for quality traits in wheat. J Cereal Sci. 2005. V. 41. P. 181-192. doi: 10.1016/j.jcs.2004.09.002

Schwarz G., Felsenstein F.G., Wenzel G. Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat. Theor Appl Genet. 2004. V. 109. P. 1064-1069. doi: 10.1007/s00122-004-1718-5

Liu S., Chao S., Anderson J. New DNA markers for high molecular weight glutenin subunits in wheat. Theor Appl Genet. 2008. V. 118. P. 177-183. doi: 10.1007/s00122-008-0886-0

Boisson M., Mondon K., Torney V., Nicot N., Laine A.L., Bahrman N., Gouy A., Daniel-Vedele F., Hirel B., Sourdille P., Dardevet M., Ravel C., Le Gouis J. Partial sequences of nitrogen metabolism genes in hexaploid wheat. Theor. Appl. Genet. 2005. V. 110(5). P. 932-940. doi: 10.1007/s00122-004-1913-4

Panchenko I.A., Usova Z.V., Prytula N.M. ta in. Informatsiyna tsinnist' ta uspadkuvannia alel'nykh variantiv blokiv vysokomolekuliarnykh hliuteniniv v selektsii ozymoi pshenytsi na iakist' zerna. Selektsiia i nasinnytstvo. 2007. No. 94. P. 115-128. [in Ukrainian]