Реакции клеточных культур кукурузы на действие жёстких осмотических стрессов
Aim. There are corn cell cultures, obtained from plants of maize inbred line L-390 (control) and from T2 progeny (L-390-T) of plants transformed via in planta Agrobacterium-mediated transformation with LBA4404 strain harboring pBi2E with double-stranded RNA-suppressor of the proline dehydrogenase gene. The reactions of cell variants, cultivated under hard osmotic stress pressure were investigated. Methods. 25.0 g/l of sea water salts or 0.8 M of mannitol were added to F1 cultural medium. Corn cell variants were tested under osmotic stress pressure. The free proline and protein levels were estimated on 14-th and 34-th days of the experiment. Results. L-390-T cell cultures maintained viability and wild type cultures died at the end of experiment. The levels of free proline rose in calli tissues, cultivated on nutrition medium with the addition of mannitol or salinity. At the same time the proline levels of L-390-T cells were products of biosynthesis. While the proline content in control cultures elevated after the degradation of proline rich proteins (PRPs). Conclusions. The L-390-T high level of osmotic stress tolerance is a possible result of transgene activity.
Keywords: Zea mays, cell cultures, Agrobacterium-mediated transformation salinity, water stress, proline.
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