The analysis of the effectiveness of the sunflower (Helianthus annuus L.) Agrobacterium-mediated transformation in planta using strain LBA4404 harboring plasmid pBi2E with double-stranded proline dehydrogenase gene RNA-suppressor

  • A. G. Komisarenko
  • S. I. Myckhalskaya
  • N. I. Adamenko
  • E. N. Tishchenko

Abstract

Aims. The effectiveness of (Helianthus annuus L.) transformation in planta using strain LВА4404 harboring plasmid pBi2E with dsRNA-suppressor (double sequence RNA-suppressor) of proline dehydrogenase gene and selective neomycin phosphotransferase II gene (nptII) was analyzed. Methods. Agrobacterium-mediated genes transfer in planta during sunflower’s pollination. Results. Seeds, RCR-analysis of which confirmed availability pro1 gene exon, of T0- and T1- sunflower’s plants have been obtained. Conclusions. It has been shown the possibility of stable integration of the transgene at the sunflower’s genome under Agrobacterium-mediated transformation in planta.

Key words: Helianthus annuus L., Agrobacterium-mediated transformation in planta.